Core--Transgenic/targeted mutagenesis

描述：（申请人描述）越来越多的转基因和敲除小鼠被用于癌症研究，以模拟基因扩增和模拟肿瘤抑制基因功能的丧失。此外，这些试剂正在成为测试新型化学预防或化疗药物的高度相关系统。转基因/靶向诱变核心的首要目标是站在转化性癌症研究的前沿。因此，在应用于临床研究之前，转基因和基因敲除小鼠是开始于细胞培养研究的逻辑和必要的延伸。该核心设施的总体目标是为加州大学旧金山癌症中心生产转基因创始和敲除嵌合体小鼠。此外，该中心还将为拥有内部专业知识的实验室提供“模块化”服务，如胚胎再分化、胚胎冷冻和用于基因靶向的标准化胚胎干细胞。此外，核心将致力于优化新兴的小鼠基因操作技术，如嵌合体生产的聚集技术，以及用于诱导基因表达和组织特异性敲除的标准化转基因系的生产。

Description: (Applicant's Description) Increasingly both transgenic and knockout mice are being used in cancer research to model gene amplification and emulate loss of tumor suppressor gene function. Moreover these reagents are emerging as highly relevant systems to test novel chemopreventive or chemotherapeutic drugs. An overriding goal of the Transgenic/Targeted Mutagenesis Core is to be at the forefront of translational cancer research. As such transgenic and knockout mice are logical and necessary extensions of investigations begun in cell culture, prior to applications to clinical investigations. The overall goals of this Core Facility will be production of transgenic founder and knockout chimera mice for the University of California San Francisco Cancer Center. In addition the Core will provide "modular" services such as embryo rederivation, embryo freezing, and standardized embryonic stem cells for gene targeting for laboratories with in-house expertise. Moreover the Core will be dedicated to optimizing emerging techniques of murine genetic manipulation such as aggregation techniques for chimera production, and production of standardized transgenic lines for both inducible gene expression, and tissue specific knockouts.