CONDUCTING POLYMER ENZYME BASED BIOSENSOR FOR NITRIC OXIDE

基于导电聚合物的微型传感器将被构建用于 一氧化氮、葡萄糖、酒精等的测定 聚合物如多金属酞菁(PmePc)和 多金属磷脂(PmePr)，可催化氧化 包括NO、NADH、H_2O_2等在内的化合物将被电化学 沉积在碳纤维(或微型碳盘)表面 电极)用作基础电极。阳离子交换剂Nafion 将在PmePc(或PmePr)涂覆的碳纤维(或 盘)电极，以区分倾向于 表现得像干预者。光电偶的表征和校准 这些微型传感器将通过循环伏安法(CV)进行， 差示脉冲伏安法、安培法、色安法、 差示脉冲安培法(DPA)等。一氧化氮传感器 将在活体内用于测量基础NO音调以及 刺激和抑制大鼠主动脉和肾动脉的NO张力 老鼠。这些实验将与不生产(或 抑制)与慢性肾功能衰竭、衰老、 环孢素肾毒性等。我们还计划建立 基于色氨酸的电化学胺传感器 含有色氨酸(TTQ)的藜麦蛋白，如甲胺 脱氢酶(MADH)和芳香胺脱氢酶(AADH)。为 这种类型的胺传感器、介体四氰基喹甲烷等 (TCNQ)将被吸附到碳盘电极表面 在胺脱氢酶被结合到表面之前 多个电极。这些传感器将被表征为 多巴胺、组胺和其他主要脂肪族和 用安培法测定芳香胺等。

Conducting polymer based micro sensors will be constructed for the determination of nitric oxide, glucose, alcohol etc. Such conducting polymers as polymetallophthalocyanines (PmePc) and polymetallophorphirins (PmePr), which can catalize the oxidation of compounds including NO, NADH, H2O2 etc., will be electro-chemically deposited on the surface of carbon fibers (or micro carbon disk electrodes) used as a base electrode. The cation exchanger Nafion film will be formed on the PmePc (or PmePr) coated carbon fiber (or disk) electrode in order to discriminate anionic species which tend to behave as interferant. The characterization and calibration of these micro sensors will be carried out by cyclic voltammetry (CV), differential pulse voltammetry (DPV), amperometry, chromoamperometry, differential pulse amperometry (DPA) etc. The nitric oxide sensors will be used in vivo to measure the basal NO tone as well as stimulated and inhibited NO tone in the aorta and renal arteries of rats. These experiments will be related to NO production (or inhibition) associated with chronic renal failure, aging, cyclosporine nephrotoxicity etc. We also plan to construct electrochemical amine sensors which are based on tryptophan tryptophylquinone (TTQ)-containing quinoproteins such as methylamine dehydrogenase (MADH) and aromatic amine dehydrogenase (AADH). For this type of amine sensor, mediators such as tetracyanoquinodimethane (TCNQ) will be absorbed to the surface of carbon disk electrode before amine dehydrogenase enzymes are incorporated onto the surface of electrodes. These sensors will be characterized for the determination of dopamine, histamine, and other primary aliphatic and aromatic amines, etc. by using amperometry.