CORE--MUTANT RODENT RESOURCE

在之前的赠款期间，研究越来越集中在 转基因和突变动物在肿瘤研究中的应用 发展。变种啮齿动物资源，一项新的共享服务，将 为McArdle科学家提供操纵 通过提供对以下内容的便捷访问，实验动物 技术： 。原核显微注射法制备转基因小鼠和大鼠 。显微注射胚胎干细胞产生嵌合体小鼠 进入寄主胚泡 。小鼠精子的冷冻保存

Research over the preceding grant period has focused increasingly on the use of transgenic and mutant animals for our studies of neoplastic development. The Mutant Rodent Resource, a new shared service, will provide McArdle scientists with the capacity to manipulate the genome of experimental animals by providing ready access to the following technologies: . Generation of transgenic mice and rats by pronuclear microinjection . Generation of chimeric mice by microinjection of embryonic stem cells into host blastocysts . Cryopreservation of mouse sperm