CORE--PATHOLOGY

病理学核心，由四名病理学教员和一名工作人员组成 助理，通过为所有计划项目提供服务 解剖病理资料收集和广泛的专业解剖 病理学及其他诊断性咨询分析。这个核心 管理以下各项的收集、记录、存储、处理和分析 生物组织标本及其识别数据和跟踪 这笔赠款。该股协调所有组织标本相关数据 收集并分发给病理学和其他弗雷德·哈钦森 癌症研究中心(FHCRC)研究实验室。骨髓, 例如，被分成血液学、病理学、 细胞遗传学、流式细胞术等实验室。长期的 随访标本由经验独特的病理学观察。 工作人员无论账单是否可以收款。处理包括 冷冻、固定、包埋、储存和适当分配给 FHCRC内部实验室的一个子集。可用的分析方法 病理学内包括研究免疫组织化学、原位 聚合酶链式反应(Y染色体DNA)杂交 (聚合酶链式反应)、荧光原位杂交(FISH)和特殊的嫁接- 抗宿主病(GVHD)研究(例如，细胞凋亡的TUNEL染色) 在人类和狗的骨髓移植受者身上。研究和 自动化免疫组织化学新方法学的发展， 原位杂交和聚合酶链式反应为 支持计划内研究项目的研究 项目。该病的发病机制及诊断研究进展 骨髓移植，如移植物抗宿主病， 静脉闭塞症和其他毒性、传染病和 分子诊断学，也支持该计划的个别项目 以及整个项目。

The pathology core, consisting of four pathology faculty and a staff of assistants, serves all of the program projects by providing anatomic pathology data collection and extensive professional anatomic pathology and other diagnostic consultative analysis. This core manages the collection, logging, storage, processing and analysis of biological tissue specimens and their identifying data and tracking for this grant. The unit coordinates all tissue specimen related data collection and distribution to pathology and other Fred Hutchinson Cancer Research Center (FHCRC) research laboratories. Bone marrow, for example, is divided into specimens for hematology, pathology, cytogenetics, flow cytometry, and other laboratories. Long-term follow-up specimens are seen by the uniquely experienced pathology staff whether billing is collectable or not. Processing includes freezing, fixation, embedding, storage, and appropriate distribution to a subset of intramural FHCRC laboratories. Analytic methods available within pathology include research immunohistochemistry, in situ hybridization (as for Y chromosome DNA), polymerase chain reaction (PCR), fluorescence in situ hybridization (FISH), and specialized graft- versus-host disease (GVHD) studies (e.g., Tunel stain for apoptosis) in human and canine marrow graft recipients. Research and development of new methodology in automated immunohistochemistry, in situ hybridization, and PCR provide "cutting edge" technology to support the studies of the research projects within the program project. Research in the pathogenesis and diagnosis of the diseases of bone marrow transplantation, such as graft-versus-host disease, venocclusive disease and other toxicity, infectious disease, and molecular diagnostics, also support the program's individual projects and the program as a whole.