CONTINUING MEGABASE SEQUENCING AT THE BCM HGSC

贝勒医学院人类基因组测序中心（BCM-HGSC） 将应用一个可扩展的，高通量的DNA测序过程， 为产生完整的人类基因组 参考序列，以及实现NIH/DOE人类 基因组计划五年计划 在第一年的能力， 产生原始DNA测序读数，并将DNA序列整理到高 质量，将积极扩大。 增加的原始数据 生产将在很大程度上依赖于一个自动化平台， 商用设备，本地开发的机器，以及 建立了BCM-HGSC测序方案。 新Perkin-Elmer 3700 毛细管DNA测序仪将用于提高通量和整个 活动将被安置在新装修的BCM-HGSC空间。整理 将通过信息学工具的组合提高费率， 单链质粒系统和自动克隆重排。 的 测序每年将产生至少44 Mb的高质量数据 第一，第二年100 Mb/年，此后120 Mb/年。 额外 还将产生4.5-5.0 X和9.0-10 X的序列覆盖度， 以便实现超过120 Mb的额外基因组覆盖范围 在第一年。“起草”的顺序将持续到最后 到2001年，重点将转向更深入地覆盖所有 序列管道中的克隆。 到2003年底， 大约1.2 Gb的序列覆盖范围，最小为530 Mb， 高质量，其余的“接近完成”。 与拟议 提高整理效率，1.2 Gb的剩余700 Mb 也将结束。 最小计划要求完成映射 代表所有人类染色体12（120 Mb）、染色体3（240 Mb） Mb）和染色体X的部分（40 Mb）。随后的目标，在此之外 400 Mb，将取决于社区定位和测序策略。 在此期间将完成适量的鼠序列， 以及其他成功社区所产生的任何过剩产能 人类DNA测序的努力将释放BCM-HGSC资源， 进一步关注模式生物。

The Baylor College of Medicine Human Genome Sequencing Center (BCM-HGSC) will apply a scalable, high throughput DNA sequencing process in a co- operative effort for the generation of a complete human genomic reference sequence, and the fulfillment of the aims of the NIH/DOE Human Genome Project five year plan. In the first year of the capacity to produce raw DNA sequencing reads, and finishing of DNA sequence to high quality, will each be aggressively expanded. The increased raw data production will rely heavily on an automated platform that integrates commercially available devices, machines developed locally, and established BCM-HGSC sequencing protocols. New Perkin-Elmer 3700 capillary DNA sequencers will be used to boost throughput and the entire activity will be housed in newly renovated BCM-HGSC space. Finishing rates will be increased by a combination of informatics tools, a double stranded plasmid system, and automatic clone re-arraying. The sequencing will generate at least 44 Mb of high quality data in year one, 100 Mb/year in year two, and 120 Mb/year thereafter. Additional sequence coverage at 4.5-5.0 X, and at 9.0-10 X will also be generated, so that more than 120 Mb of additional genome coverage will be achieved in the first year. The sequence 'drafting' will continue until the end of 2001, when the emphasis will shift towards deeper coverage of all clones in the sequence pipeline. By the end of 2003 there will be approximately 1.2 Gb of sequence coverage, with a minimum of 530 Mb at high quality and the remainder 'near finished'. With the proposed increases in finishing efficiency, the remaining 700 Mb of the 1.2 Gb will also be finished. The minimal plan calls for completion of mapped clones representing all human Chromosome 12 (120 Mb), Chromosome 3 (240 Mb) and portions of Chromosome X (40Mb). Subsequent targets, beyond this 400 Mb, will depend on the community mapping and sequencing strategies. A modest amount of murine sequence will be completed during this time, and any excess capacity resulting from other successful community efforts for human DNA sequencing will free the BCM-HGSC resource to focus further on model organisms.