CORE--MR SPECTROSCOPY /IMAGING FACILITY

该核心的总体目标是开发和应用多核磁共振（NMR）光谱和成像核心设施，用于研究发育中大鼠的缺氧缺血性脑损伤。Core的光谱组件将应用MR光谱技术评价发育中大鼠脑缺氧缺血期间和之后发生的代谢变化。空间定位的31 P和1H MR光谱将用于定量能量代谢、乳酸盐浓度、细胞内pH值和游离细胞溶质镁浓度的变化。核心的成像部分将继续开发和应用磁共振成像（MRI）技术，以评估围产期缺氧缺血性脑损伤。两种成像技术将继续用于阐明缺氧缺血和恢复不同阶段脑损伤的部位和严重程度。这些技术包括扩散和T2加权自旋回波成像。我们以前的研究结果表明，弥散成像是一种敏感的方法，早期检测缺氧缺血性损伤，而T2加权自旋回波成像更准确地监测长期损害。动物运动和多动物设备获得可靠的图像必不可少的将不断设计，建造和完善。这些设备将被纳入光谱和成像的一般探头设计。

The overall objective of this Core is to develop and apply multi-nuclear magnetic resonance (NMR) spectroscopy and imaging core facilities for the study of hypoxic-ischemic brain damage in developing rats. The spectroscopy component of the Core will apply MR spectroscopy techniques to evaluate metabolic alterations which occur during and following cerebral hypoxia-ischemia in developing rats. Spatially localized 31P and 1H MR spectroscopy will be used to quantitate changes in energy metabolism, lactate concentrations, intracellular pH, and free cytosolic magnesium concentrations. The imaging component of the Core will continue to develop and apply magnetic resonance imaging (MRI) techniques to evaluate perinatal hypoxic-ischemic brain damage. Two imaging techniques will continue tobe used to elucidate the sites and severities of brain damage at different stages of hypoxia-ischemia and recovery. These techniques include diffusion and T2-weighted spin echo imaging. Our previous results have indicated that diffusion imaging is a sensitive method for early detection of hypoxic-ischemic injury, while T2-weighted spin echo imaging more accurately monitors long-term damage. Animal movement and multi- animal devices essential for obtaining reliable images will be continually designed, constructed and refined. These devices will be incorporated into the general probe design for spectroscopy and imaging.