CORE--BASIC SCIENCE AND ENGINEERING

基础科学和工程核心设施将实现以下目标 为老龄问题司提供的服务： 1)基因组扫描DNA标记服务将帮助老年医生 基因组序列和DNA标记鉴定的调查员 与晚年流行疾病有关的面板。 将提供专门的二维电泳仪。 2)基因鉴定和表征服务将使 老年病学家/老年病学家识别有差异的基因 在衰老和/或疾病期间表达。用于减法的设备 杂交和聚合酶链式反应差异显示技术将可用。 3)氧化DNA损伤确定服务将允许临床医生 调查人员将确定DNA氧化损伤的程度，即 在特定患者的血液或尿液中可检测到的。高性能 将可进行高效液相分析。 4)转基因动物设计服务将协助调查人员 利用显微注射DNA或基因打靶技术设计转基因小鼠 同源重组。接近微量注射设备或 将提供胚胎干细胞实验室。 5)生物数据采集服务提供记录设备，用于 步行节奏、心电图和持续血压。它还借出了 生物监测用换能器、放大器和记录器 信号。 6)生物信号处理服务数字化、显示和分析 生物信号，如通过服务#5获得的信号。特殊 用于分析步行节奏、心电图和血压的软件 以及通用软件(用于执行傅里叶变换， 滤波、主成分分析和计算非线性 将提供诊断(如Lyapunov指数)。 7)图像采集服务为以下对象提供研究级显微镜 需要高分辨率成像的细胞生物学项目。这项服务 还提供用于患者研究的摄像机和录像机 步态和动作。 8)图像处理和解释服务执行数字图像 诸如通过图像采集记录的图像的处理 服务，包括图像增强和过滤、图像分割和 显微光学序列的模式识别与三维重建 横断面。

The Basic Science and Engineering Core facility will make the following services available to the Division on Aging: 1) A Genome Scanning for DNA Markers Service will assist the geriatrician investigator with the identification of genomic sequences and DNA marker panels that are associated with the prevalent disease of late life. Specialized 2-D electrophoresis units will be provided. 2) A gene Identification and Characterization Services will enable the geriatrician/gerontologist to identify genes that are differentially expressed during aging and/or disease. Equipment for subtractive hybridization and PCR differential display techniques will be available. 3) An Oxidative DNA Damage Determination Service will allow the clinician investigator to determine the extent of oxidative DNA damage that is detectable in the blood or urine in a given patient. High performance liquid chromatography analysis will be available. 4) A Transgenic Animal Design Service will assist investigators in the design of transgenic mice using microinjection of DNA or gene targeting and homologous recombination. Access to the microinjection equipment or embryonic stem cell laboratory will be provided. 5) A Biological Data Acquisition Service provides recording devices for walking cadence, ECG and continuous blood pressure. It also loans transducers, amplifiers, and recorders for the monitoring of biological signals. 6) A Biological Signal Processing Service digitizes, displays and analyzes biological signals such as those obtained through service #5. Special software for the analysis of walking cadence, ECG, and blood pressure as well as general purpose software (for performing Fourier transforms, filtering, principal component analysis, and for calculating non-linear diagnostics such as Lyapunov exponents) will be provided. 7) An Image Acquisition Service provides research grade microscopy for cell biology projects that require high resolution imaging. This service also makes available video cameras and recorders for the study of patient gait and movement. 8) An Image Processing and Interpretation Service performs digital image processing of images such as those recorded by the image acquisition service, including image enhancement and filtering, image segmentation and pattern recognition, and 3-D reconstruction from microscopic optical serial sections.