BEHAVIORAL & PHYSIOLOGICAL CONSEQUENCES OF LOSS: SIV

已有研究表明，将非人类灵长类动物与它们的 母亲在婴儿时期的一小段时间会导致永久性的 免疫系统的变化。尚未确定的是 这种应激源引起的免疫变化是否会导致风险增加 发病率或死亡率。在这项研究中，我们正在评估疾病 20例4岁儿童接种SIVe660的进展 尾辫猕猴(Macaca Nomestrina)。这些受试者都是 科罗拉多大学的实验方案，在那里他们的 从婴儿时期起，行为和免疫发育就受到密切关注。 一半的受试者在6岁时经历了为期两周的母亲分居 几个月大，一半没有。到目前为止，已经有七只猴子 运往西雅图接种SIV疫苗。一名受试者已被感染 28周，4人感染8周。另外两个 动物目前正在接受隔离，并将在 三月。感染前在西雅图进行的抽血结果显示 分离后的动物有更高比例的 抑制性/细胞毒性T淋巴细胞(CD8+)和自然杀伤细胞 (CD16+)。他们的红细胞压积和血红蛋白也较高。 级别。感染后8周CD8+细胞比例 所有受试者的CD4+比例都增加了(辅助T 淋巴细胞)和CD20+(未激活的B淋巴细胞)减少 意义重大。受试者每周都要接受测试，测试的内容是 饮用首选液体(Kool Aid)。所有受试者都显示出 感染后前8周的消耗量显著增加； 在经历过的动物之间没有什么区别 分离的和那些没有在液体用量上的。 每周还会进行三次行为观察，以 确定感染引起的行为变化。资助美国国立卫生研究院拨款 RR00166和NIMH授予MH37373、MH19514、MH45045和MH15442。

It has been shown that separating nonhuman primates from their mothers for a short period of time as infants results in permanent alterations in the immune system. What has not been determined is whether immune changes due to this stressor result in increased risk for morbidity or mortality. In this study we are evaluating disease progression following inoculation with SIVe660 in 20 four-year-old pigtailed macaques (Macaca nemestrina). The subjects were part of an experimental protocol at the University of Colorado, where their behavioral and immune development was closely monitored since infancy. Half of the subjects experienced a two-week maternal separation at six months of age and half did not. To date seven monkeys have been shipped to Seattle for SIV inoculation. One subject has been infected for 28 weeks and four have been infected for 8 weeks. Two additional animals are currently undergoing quarantine and will be infected in March. Blood draws taken in Seatt le b efore infection showed that animals that undergwent separation had higher proportions of suppressor/cytotoxic T lympocytes (CD8+) and natural killer cells (CD16+) than controls. They also had higher hematocrit and hemoglobin levels. In the 8 weeks after infection proportions of CD8+ cells increased for all subjects while proportions of CD4+ (Helper T lymphocytes) and CD20+ (nonactivated B lymphocytes) decreased significantly. Subjects are being tested weekly on the amount of consumption of a preferred liquid (Kool Aid). All subjects showed a significant increase in consumption in the first 8 weeks of infection; there was no difference between animals that had experienced separation and those that had not in the amount of liquid consumed. Behavioral observations are also conducted three times per week to determine behavioral changes due to infection. FUNDING NIH grant RR00166 and NIMH grants MH37373, MH19514, MH45045, and MH15442.