PROPERTIES OF SINGLE ASSEMBLIES OF LIGHT HARVESTERS LH1 & LH2

我们正在研究的光漂白和转移动力学在单一的 组装的LH复合物和？二肽， 从他们身上可逆地提取出来。 每个LH2包含18个 细菌叶绿素（B850）和九个收集器（B800）。 的 荧光寿命、偏振、相关时间和漂白 在各种条件下研究动力学。 主 目标包括长时间尺度蛋白质动力学的测量。 极化实验揭示了构象动力学 与激子迁移在九聚体LH 2组装。 此外，他们还表示，单一组件不是 圆形电子，如X射线衍射所预期的 问题研究

We are examing the photobleaching and transfer dynamics in single assemblies of the LH complexes and the ??-dipeptides that can be reversibly extracted from them. Each LH2 contains eighteen bacteriochlorophylls (B850) and nine collectors (B800). The fluorescence lifetimes, polarization, correlation times and bleaching kinetics are being studied under a variety of conditions. The primary goals include measurements of long time scale protein dynamics. Polarization experiments have revealed conformational dynamics associated with excitonic migration in the nonameric LH2 assembly. Furthermore, they have indicated that the single assemblies are not circular electronically as would be expected from X-ray diffraction studies.