STRUCTURE & ASSEMBLY OF RETROVIRUSES

Rous肉瘤病毒(RSV)颗粒在体外与 GAG蛋白在大肠杆菌和大肠杆菌RNA中的表达及在 茎。它们看起来很像未成熟的逆转录病毒，并有肿块。 SES约为80mD，相当于每个约1200个Gag蛋白质 粒子。其他结构正在研究中。此外, 几个实验室开始对测量质量感兴趣。 因为其中的大多数数据大约是20年前的。一些人 对RSV的研究已经取得了初步结果。两个品系 从受感染的火鸡体内(整体)提纯病毒颗粒 细胞。一个只有Gag、Pol和Env的基因。另一种是携带 一个额外的基因(碱性磷酸酶)使基因组-20% 更大的(就像携带src癌基因的真正的RSV一样)。大小 逆转录病毒的大小似乎是不同的，一个假设是 是由包装的RNA的数量决定的。质量测量来自 STEM数据显示，MW看起来大致相同。

In vitro particles of Rous sarcoma virus (RSV) were assembled with Gag protein expressed in E. coli and E. coli RNA and examined in the STEM. They appeared very much like immature retroviruses and had ma ses of about 80 Md, corresponding to about 1200 Gag proteins per particle. Other constructions are being studied. In addition, several laboratories are becoming interested in measuring the masses of retroviruses since most data bn them is about 20 years old. Some preliminary results for RSV have been obtained. Two strains of (whole) in vivo virus particles were purified from infected turkey cells. One has a genorne of only Gag, Pol, and Env. The other carries an additional gene (alkaline phosphatase) making the genome -20% larger (like the "real" RSV which carries the src oncogene). The size of retroviruses appears variable and one hypothesis is that the size is determined by the amount of RNA packaged. Mass measurements from the STEM data indicate that the MWs appear about the same.