HCG LH/CG RECEPTOR BINDING AND ACTIVATION

描述：(改编自申请人的摘要)这项工作的目的 项目是对导致以下问题的结构决定因素的阐明 人绒毛膜促性腺激素与其受体的特异性高亲和力结合及其机制 配体介导的受体激活。主要有两个目标，即1) 激素和受体结合决定因素的描述及2)阐明 受体激活和跨膜信号转导的机制。 人绒毛膜促性腺激素α亚基、人绒毛膜促性腺激素β和黄体生成素/卵泡刺激素受体的定点突变 将用于检验hCG和hCG的离散区域的假设 受体负责高亲和力结合。调查将会 利用激素受体多肽融合技术构建 推测是迷你促性腺激素。将利用受体突变研究 为了检验跨膜螺旋6和7含有氨基酸的假设 在信号传递中很重要但在配体结合中不重要的残基。

DESCRIPTION: (adapted from the applicant's abstract) The objective of this project is the elucidation of the structural determinants leading to specific high-affinity binding of hCG to its receptor and the mechanism of ligand-mediated receptor activation. There are two major aims, namely 1) Delineation of hormone and receptor binding determinants and 2) Elucidating the mechanism of receptor activation and transmembrane signaling. Site-directed mutagenesis of alpha subunit, hCG-beta, and LH/CG receptor will be used to test the hypothesis that discrete regions of hCG and of receptor are responsible for high-affinity binding. The investigations will exploit a hormone-receptor polypeptide fusion technique to construct a putative mini-gonadotropin. Receptor mutagenesis studies will be utilized to test the hypothesis that transmembrane helices 6 and 7 contain amino acid residues that are important in signaling but not ligand binding.