CORE--LIPID/PEPTIDE PROBE FACILITY

目的该核心将为该计划内的子项目提供化学合成服务和探测器开发方面的专业知识。大部分服务组件将发展为多肽和笼状化合物的合成、纯化和分析。将在商业笼状化合物的使用方面提供技术援助，包括化合物纯度的色层分析，以及在特定实验条件下确定光转化程度。次级项目中使用的许多化合物没有商业用途，必须定期制备。在某些情况下，化合物需要被放射性标记，或者附加生物素或荧光素，以解决关于化合物的稳定性、局部化或细胞内有效浓度的特定问题。标签和以其他方式为特定目的定制试剂的灵活性代表了拥有内部化学合成能力的许多显著优势中的两个。核心工作人员将与分项目人员协商，根据正在调查的生理或生化系统的要求，设计和实施综合策略。

Purpose This core will provide chemical synthesis services and expertise in probe development for subprojects within the Program. The majority of the service component will be developed to synthesis, purification, and analysis of peptides and caged compounds. Technical assistance in the use of commercial caged compounds will be provided including chromatographic analysis of compound purity, and determination of extent of photo-conversion under specific experimental conditions. Many of the compounds being used in the subprojects are not commercially available and mus be prepared on a regular basis. In some instances, compounds need to be radiolabeled, or have biotin or fluorophores attached for addressing specific issues regarding a compounds stability, localization, or effective concentration in cells. Labeling and the flexibility to otherwise customize reagents for specific purposes represent two of many significant advantages of having 'in house' chemical synthesis capabilities. Core staff in consultation with subproject personnel will design and implement synthetic strategies based upon the requirements of the physiological or biochemical system under investigation.