EXTENSIONS OF MICROMACHINED MICROELECTRODE TECHNOLOGY

本项目旨在扩展基本的无源探头 技术，使探针更通用， 与周围的细胞相互作用。 我们已经开发并 成功地实施了一个过程，允许包括 化学输送通道在与导电层相同的基底上 电极部位。 这允许多点药物输送， 多通道记录和/或刺激 破坏 化学品运输的成功包装技术 已经开发了允许在正常环境中操作和使用的探针 神经元急性药理学表征背景 活动 体外试验证实了定量的 剂量的流体到周围介质。 体内试验同时 豚鼠下丘脑的化学传递和细胞外记录 和上级丘的结果与 传统的方法。 在会议上提出的数据，包括 1998年耳鼻喉科学研究协会 神经科学界对该设备的兴趣。 新设备 已经制造了具有与特定的 神经生理学研究将在布莱索博士的 实验室 过去一年的发展包括： 用于监测输送的化学品剂量的在线流量计。

This project is designed to extend the basic passive probe technology to make the probes more versatile by giving them more ways to interact with the cells which surround them. We have developed and successfully implemented a process which allows the inclusion of chemical delivery channels on the same substrate as the conductive electrode sites. This permits multipoint drug delivery and multichannel recording and/or stimulation with minimal tissue disruption. Successful packaging techniques for the chemical delivery probes have been developed which permit handling and use in the normal context of acute pharmacological characterization of neuronal activity. In vitro tests have verified the passage of quantified doses of fluid to a surrounding medium. In vivo tests of simultaneous chemical delivery and extracellular recordings in guinea pig inferior and superior colliculus are demonstrating results consistent with conventional approaches. Data presented at meetings including that of the 1998 Association for Research in Otolaryngology has generated much interest in the device by the neuroscience community. New devices have been fabricated which have configurations relevant to specific neurophysiological studies to be carried out in Dr. Bledsoe's laboratory. Developments during the past year include the development of an in-line flowmeter for monitoring of the dose chemical delivered.