LEICS TCS SP2 CONFOCAL LASER SCANNING MICROSCOPE

该提案是为Forsyth研究所的生物结构核心设施获得一个徕卡TCS SP2共聚焦激光扫描头，安装在一个直立DM RXE显微镜（CLSM）上，具有一系列水、空气和油物镜，用氩离子和两个He/Ne激光器发射。包括一个卫星图像分析中心。福赛斯是世界上最重要的口腔和颅面生物学独立研究机构。为了保持高水平的成就，是时候扩展现有的仪器了。几个受资助者目前将时间外包给不同的CLSM，两个受资助者依赖于CLSM的使用，两个最近发表了使用CLSM的文章。获得CLSM理想地增强了福赛斯的四个广泛的科学兴趣。福赛斯的几位研究人员正在研究口腔生物膜的形成，生物膜与上皮细胞的相互作用，或对生物膜的免疫反应。CLSM是研究生物膜细菌组成的首选仪器。(2)福赛斯的其他人正在研究人类颅面突变中的特定基因表达、蛋白质表达和分子相互作用。福赛斯的斑马鱼设施是胚胎的来源，用于研究与正常和突变颅面软骨分化有关的基因和蛋白质。CLSM的光学切片能力是定位用于标记细胞和组织中的基因和蛋白质的荧光探针的理想选择。(3)在Forsyth进行了细胞粘附分子、细胞骨架和牙釉质基质蛋白在生物矿化中的相互作用的基础研究。(4)另一个关注的焦点是通过定位破骨细胞中的基因表达、蛋白质加工和分子相互作用来探索骨吸收中的基因调控机制。CLSM旨在适应处理这些研究方法的技术。CLSM的使用将在超过12名研究者、他们的博士后研究员以及来自东北大学和哈佛大学牙科医学院的科学家之间共享。福赛斯CLSM监督委员会将监管CLSM使用的各个方面。福赛斯研究所保证提供：1)所有必要的CLSM房屋翻新，2)仪器寿命的服务合同，3)应用支持，以及4)训练有素的操作人员为CLSM的所有用户提供服务。

This proposal is for the Biostructure Core Facility of The Forsyth Institute to obtain a Leica TCS SP2 Confocal Laser Scanning head mounted on an upright DM RXE microscope (CLSM) with a series of water, air, and oil objectives, fired with Ar ion and two He/Ne lasers. A satellite image analysis center is included. Forsyth is the worlds foremost independent research institute in oral and craniofacial biology. In order to maintain high standards of achievement it is time to expand existing instrumentation. Several of the grantees currently outsource time on different CLSMs, two grants depend on CLSM use, and two have recent publications using CLSM. Obtaining a CLSM ideally enhances four broad scientific interests at Forsyth. (1) Several investigators at Forsyth are studying oral biofilm formation, the interaction of biofilm with epithelial cells, or the immune response to biofilm. The CLSM is the instrument of choice for the study of the bacterial composition of biofilm. (2) Others at Forsyth are studying specific gene expression, protein expression and molecular interactions in craniofacial mutations occurring in humans. The Zebrafish facility at Forsyth is a source of embryos used to study genes and proteins involved in normal and mutant craniofacial cartilage differentiation. The optical sectioning capability of the CLSM is ideal for locating fluoroprobes used to mark genes and proteins in cells and tissues. (3) Basic research is conducted at Forsyth in the interaction of cell adhesion molecules, the cytoskeleton, and enamel matrix proteins in biomineralization. (4) Another focal interest is exploring mechanisms of gene regulation in bone resorption by localizing gene expression, protein elaboration and molecular interactions in osteoclasts. The CLSM is designed to accommodate techniques that address these research approaches. Use of the CLSM will be shared among more than 12 investigators, their post-doctoral fellows, and scientists from Northeastern University and Harvard School of Dental Medicine. The Forsyth CLSM Oversight Committee will regulate all aspects of CLSM use. The Forsyth Institute guarantees to provide: 1) all necessary renovations for housing the CLSM, 2) the service contract for the life of the instrument, 3) applications support, and 4) a trained operator to be available for all users of the CLSM.