PURCHASE OF 600MHZ NMR SPECTROMETER

申请600兆赫核磁共振光谱仪的资金，以支持一组NIH和NSF资助的研究人员在加州大学洛杉矶分校的研究。光谱仪将位于加州大学洛杉矶分校-美国能源部大分子核磁共振设施。该高场核磁共振波谱仪将用于帮助解决主要用户提出的蛋白质、核酸、dna -蛋白质、rna -蛋白质和蛋白质-蛋白质复合物的结构。五个主要用户的研究项目描述如下：(1)核酸和复合物的结构研究（Feigon），(2)蛋白质及其DNA复合物的结构研究（Clubb）， (3) Vpr相关蛋白的结构/功能研究（Chen）， (4) DNA建筑蛋白的结构研究（Johnson），(5)嗜气焦杆菌和结核分枝杆菌蛋白溶液的结构研究（Eisenberg）。为了给主要用户提出的项目提供足够的高场核磁共振时间，也为了让额外的次要用户更多地使用高场核磁共振光谱仪进行大分子研究，迫切需要这种光谱仪。

Funding is requested for a 600 MHz NMR spectrometer to support the research of a group of NIH and NSF funded researchers at UCLA. The spectrometer will be located in the UCLA-DOE macromolecular NMR facility. This high field NMR spectrometer will be used to help solve the structures of proteins, nucleic acids, DNA-protein, RNA-protein, and protein-protein complexes proposed for investigation by the major users. The research projects of the five major users are described with the following titles: (1) Structural studies of nucleic acids and complexes (Feigon), (2) Structural studies of proteins and their DNA complexes (Clubb), (3) Structure/Function of Vpr Associated Proteins (Chen), (4) Structural studies of DNA architectural proteins (Johnson), and (5) Structural studies in solution of proteins from pyrobaculum aerophilum and mycobacterium tuberculosis (Eisenberg). This spectrometer is urgently needed to provide adequate high field NMR time for the projects proposed by the major users, as well as to allow additional minor users more access to high field NMR spectrometers for macromolecular studies.