PURCHASE OF A FINNIGAN LCQ DECA INTEGRATED LCMS SYSTEM

我们要求购买一台集成的超灵敏微孔液相色谱-质谱仪，该仪器具有蛋白质电喷雾电离分析和蛋白质酶促分析的能力。所要求的仪器是Finnigan LCQ Deca，能够在亚皮摩尔至飞摩尔水平上对肽进行质谱裂解和测序。它在我们现有的MS核心设施的存在将增加我们目前缺乏的能力，以支持PI和其他六名教师的研究计划，这些研究计划将通过攻击问题的能力得到显着增强，这些问题目前是不可能与校园内现有的设备。该仪器具有分析和测序的能力，即使是发生在反相液相色谱上的多个肽片段的混合物。这将有利于一些NIH资助的研究项目，处理各种健康相关的问题。

We request purchase of an integrated ultra-sensitive microbore liquid chromatograph-mass spectrometer with capabilities for electrospray ionization analysis of proteins and enzymatic digests of proteins. The instrument requested, a Finnigan LCQ Deca, is capable of mass spectral fragmentation and sequencing of peptides at sub-picomole to femtomole levels. Its presence in our existing MS Core Facility will add capabilities we currently lack to support the research programs of the PI and six other faculty with research programs which will be dramatically enhanced by the ability to attack problems which are currently not possible with existing equipment on campus. This instrument has the capability of analyzing and sequencing even mixtures of multiple peptide fragments that happen to co-elute on reversed-phase liquid chromatography. This will benefit a number of NIH-funded research programs dealing with a variety of health-related problems.