FINNIGAN LCQ DECA QUADRUPLE ION TRAP MASS SPECTROMETER

请求提供资金购买Finnigan LCQ（DECA）四极离子阱质谱仪。作为该机构的第一台质谱仪，它将在扩大潜在的研究领域方面产生巨大的影响。十个主要用户对该仪器的拟议使用涵盖了广泛的医学研究领域。肌肉相关项目包括肌球蛋白甲基化的潜在作用研究（用户1），以及骨骼肌发育和心脏发生中调节蛋白/DNA相互作用的研究（用户2）。与转录和翻译有关的项目通常有三个项目：延长阻滞因子GreA和GreB与RNA聚合酶的相互作用（用户3），模型mRNA，RNA结合真核起始因子和核糖体蛋白之间相互作用的表征（用户4），以及RNA聚合酶，DNA模板和转录过程中新生RNA之间相互作用的表征（用户5）。细胞信号传导项目出现在一项新描述的脑蛋白激酶C形式的起源、磷酸化状态和功能的研究中（用户6），在长期暴露于吗啡后腺苷酸环化酶磷酸化状态的分析中（用户7），以及在酵母细胞决定选择减数分裂或有丝分裂期间RAN 1激酶的作用和调节的磷酸化研究中（用户8）。海马和纹状体中NMDA受体调节的神经递质释放的研究将在自由行为动物中结合联合收割机单细胞记录和微透析技术（用户9）。用户10将研究发育期可卡因暴露对大鼠选定脑区神经肽水平的影响。该应用程序是由最近招聘的一名教师在质谱丰富的经验支持。

Funds are requested to purchase the Finnigan LCQ(DECA) Quadrupole Ion Trap Mass Spectrometer. As the first mass spectrometer at the institution, it will have an enormous impact in its expansion of potential areas for investigation. The proposed use of the instrument by ten major users covers a wide range of medically oriented research areas. These may be summarized as follows: Muscle-related projects include the study of a potential role for methylation of myosin (User 1), and regulatory protein/DNA interactions in skeletal muscle development and cardiogenesis (User 2). Projects relating to transcription and translation in general are featured in three projects: the interaction of elongation arrest factors GreA and GreB with RNA polymerase (User 3), the characterization of interactions between model mRNAs, RNA-binding eukaryotic initiation factions and ribosomal proteins (User 4), and characterization of the interactions between RNA polymerase, the DNA template, and nascent RNA during transcription (User 5). Cell signaling projects appear in a study of the origin, phosphorylation state and function of a newly described form of brain protein kinase C (User 6), in an analysis of the phosphorylation state of adenylyl cyclase following chronic exposure to morphine (User 7), and in a phosphorylation study of the action and regulation of RAN1 kinase during the decision by yeast cells to select meiosis or mitosis (User 8). A study of NMDA receptor-regulated neurotransmitter release in hippocampus and striatum will combine single-cell recording and microdialysis techniques in freely behaving animal (User 9). The effects of developmental cocaine exposure on neuropeptide levels in selected brain regions of rats will be investigated by User 10. The application is supported by the recent recruitment of a faculty member with extensive experience in mass spectrometry.