PE BIOSYSTEMS VOYAGER DE MALDI-TOF

申请资金用于购买Voyager-DE生物光谱分析仪工作站。该系统采用蛋白质、肽和寡核苷酸为辅助基质。该仪器的强大之处在于延迟提取技术。通过减小背景和提高信噪比，获得了显著的质量分辨率。因此，对各种来源的复杂成熟蛋白和脆弱分子（如DNA）的分析可以非常准确地完成。由于背景减小，该软件能够缩小参数范围，并显著减少进行有效分配所需的脉泽数量。该软件还允许通过搜索虚拟消化数据库来识别蛋白质。该系统具有通用性、扩展性和易于操作的特点，是多个运营商和项目的理想选择。我们的具体应用包括：c端和n端蛋白和寡核苷酸测序，鉴定未知蛋白和确定跨国修饰。样品可以从凝胶、PVDF膜、TLC板和/或Biacore芯片等来源中提取。12项研究建议中详细描述的具体目标的实现，将由Voyager-DE MALDI-TOF提供便利

This request is for funds to purchase a Voyager-DE Biospectrometry Workstation. This system uses matrix-assisted of proteins, peptide and oligonucleotide. The power of this instrument lies in the delayed extraction (DE) technology. Significant mass resolution is obtained by reducing the background and increasing the signal to noise ratio. Therefore analyses of complex protein matures from various sources and fragile molecules, such as DNA, can be accomplished with great accuracy. Because of the decreased background the software is able to narrow the parameters and significantly reduce the number of masers necessary to make valid assignments. The software also allows identification of proteins by searching a virtual digestion database. This system is ideal for multiple operators and projects beaus of its versatility, spread and ease of operation. Our specific applications include: C-terminal and N-terminal protein and oligonucleotide sequencing, identifying unknown proteins and determining post-transnational modifications. Samples can be extracted from such sources as gels, PVDF membranes, TLC plates, and/or Biacore chips. The fulfillment of the specific aims described in detail in the twelve research proposals would be facilitated by the addiction of a Voyager-DE MALDI-TOF