BIOROBOT 3000

描述：(调查员？S摘要)：此应用程序请求资金 购买一个液体处理和核酸制备机器人，代表 构建卓越的基因治疗核心设施的协调计划的一部分 和康奈尔大学威尔医学院的基因表达分析 大学。这些共享设施将为康奈尔大学和附属教职员工提供 利用阵列方法分析基因表达的技术和 基因治疗技术从基础研究到临床试验。基于 基因治疗核心初始使用者的时间利用研究 设施，我们已经确定了分子的常规重复操作 生物学使用了大量熟练的技术人员的时间，而且 大量使用时容易出错。BioRobot 3000代表着 灵活且多功能的系统，可供多个 研究人员用于高通量和重复性的样品制备。一个 由具有广泛研究的五名主要或三名次要调查人员组成的小组 支持，主要来自NIH来源，已被确定为初始用户 基因表达基因治疗的核心和代表了 设备。其中预期的应用包括常规制备质粒 从体外细胞培养和体内制备DNA、基因组DNA和RNA 基因转移的研究和聚合酶链式反应的建立。在基因的背景下 表达核心、质粒制备及后PCR清理应用 将被用来提供点阵的原材料。总体而言 基因治疗核心设施的组织结构和设施 提供地点、永久管理和监督机制，以 确保仪器的最佳使用。

DESCRIPTION: (Investigator?s Abstract): This application requests funds to purchase a liquid handling and nucleic acid preparation robot that represents part of a coordinated plan to build a superior Core Facilities for Gene Therapy and Gene Expression analysis at The Weill Medical College of Cornell University. These shared facilities will provide Cornell and affiliated faculty with the technology to analyze gene expression by array methods and the technology of gene therapy from basic research to clinical trials. Based on time utilization studies of the initial users of the Gene Therapy Core Facility, we have determined that routine repetitive operations of molecular biology use an inordinate amount of the time of skilled technicians and are prone to error when done in large numbers. The BioRobot 3000 represents a flexible and versatile system that can readily be used by a number of researchers for sample preparation with high throughput and reproducibility. A group of five major or and three minor investigators with extensive research support, primarily from NIH sources, have been identified as the initial users of the gene expression gene therapy cores and represent the users of the equipment. Among the intended applications are routine preparation of plasmid DNA, genomic DNA and RNA preparation from in vitro cell culture and in vivo studies of gene transfer, and PCR reaction setup. In the context of the gene expression core, the plasmid preparation and post-PCR clean up applications will be used to provide raw material for spotting arrays. In the overall organizational structure and facilities of the Gene Therapy Core Facility provide the location, permanent management and oversight mechanisms that ensures the optimal use of the instrument.