CORE--CELL BIOLOGY

该计划项目代表了一组具有凝聚力的实验室和 拥有共同目标和战略的调查人员，专注于 腺病毒介导的呼吸道基因治疗作为一种治疗方法 治疗囊性纤维化的治疗工具。这些实验室还 共享对几种技术的需求，包括形态分析 通过基因转移研究和专业技术产生的数据 生长处理，并分析基因与之相关的细胞培养 调走了。细胞生物学核心为研究人员提供了一种 多种成像和检测方法及特殊细胞培养 研究载体与靶细胞、表达载体相互作用的技术 载体编码的转基因，以及转基因的生理影响 表情。为此，细胞生物学核心保持了 最先进的荧光和明场图像采集设备， 文档、演示文稿和促进的ACE到其他 医疗中心资源，如组织学、超微结构和 共焦设施。细胞生物学核心的工作人员拥有广泛的 接受各种细胞学、细胞培养技术和 将投入时间和资源将这些资产提供给 参与项目。

This Program Project represents a cohesive group of laboratories and investigators that share common goals and strategies focussing on the use of adenovirus-mediated gene therapy to the respiratory tract as a therapeutic tool for treating cystic fibrosis. These laboratories also share a need for several techniques including morphological analysis of data generated by gene transfer studies and specialty techniques for growing handling, and analyzing cell cultures to which genes are transferred. The Cell Biology Core provides to the investigators a variety of imaging and detection methods and specialty cell culture techniques to study interaction of vectors with target cells, expression of vector-encoded transgenes, and the physiological impact of transgene expression. Toward this end, the Cell Biology Core maintains state-of- the-art equipment for fluorescence and brightfield image acquisition, documentation, and presentation and facilitated aces to additional medical center resources such as histological, ultrastructural, and confocal facilities. The staff of the Cell Biology Core has extensive training in a variety of cytological, and cell culture techniques and will dedicate time and resources to make those assets available to the participating projects.