EFFECTS OF CARBOPLATIN AND KAINIC ACID ON EVOKED RESPONSES

提出了一系列研究功能的实验。 永久性内毛细胞(IHC)丢失的后果 卡铂或使用红藻氨酸的八种神经纤维的暂时性变性 酸。实验动物将是栗鼠。初步数据 从这个实验室展示了唤起的不同模式的变化 听觉神经系统不同位置的潜在波幅 在注射卡铂或应用海人酸后， 圆窗。我们将通过记录来调查外毛细胞的完整性 每个根管早期的失真产物耳声发射 动物。在基线研究之后，我们将评估以下时间进程 卡铂和红藻氨酸对反应的功能影响 来自第八神经、下丘和听觉皮质。使用这个 方法，我们将研究IHC功能对听觉的作用 功能，将研究听觉系统的功能重组 以应对永久性的IHC丢失和暂时性的去传入。几个 实验将使用GABA阻滞剂来检验这一假设 去抑制在中枢神经系统的代偿性变化中起作用 外周损伤后的听觉结构。一个基本的假设 这项工作的一个特点是，外周和中枢效应的分离 耳毒性侮辱，或不同的康复过程是证据 补偿性职能重组。

A series of experiments are proposed which investigate the functional consequence of either permanent inner hair cell (IHC) loss using carboplatin or temporary degeneration of eight nerve fibers using kainic acid. The experimental animal will be the chinchilla. Preliminary data from this laboratory demonstrate different patters of changes in evoke potential amplitude at varying locations in the auditory nervous system following injection of carboplatin or application of kainic acid to the round window. We will survey outer hair cell integrity by recording distortion product otoacoustic emissions from the early canal of each animal. Following baseline studies, we will evaluate the time course of the functional consequence of carboplatin and kainic acid on the responses from the eighth nerve, inferior colliculus and auditory cortex. Using this approach, we will investigate the role of IHC function on auditory function and will study functional reorganization in the auditory system in response to permanent IHC loss and transient deafferentation. Several experiments will use GABA blockers to test the hypothesis that disinhibition plays a role in compensatory changes seen in central auditory structures following peripheral damage. A fundamental assumption of this work is that a dissociation of peripheral and central effects of ototoxic insults, or a differing time course of recovery is evidence of compensatory functional reorganization.