CORE--MONOCLONAL ANTIBODY PRODUCTION FACILITY

抗体生产核心(APC)旨在提高效率 三个计划项目实验室中的所有阶段的克隆 抗体的产生和纯化。APC主任将协助 调查人员在适当的动物免疫中，收集 免疫血清，并提供可能的抗体筛查信息 面向项目科学家的战略。细胞融合及其后续克隆 而特定杂交瘤的冷冻保存将由 华盛顿大学医学院杂交瘤中心。一旦 项目科学家已经确定了所需的杂交瘤 抗体生产核心将产生含有抗体的上清液， 纯化这些单抗并提供给计划项目 实验室提纯量的10株单抗 杂交瘤细胞的升培养。

The Antibody Production Core (APC) is designed to improve the efficiency of the three Program Project laboratories in all phases of monoclonal antibody production and purification. The APC Director will assist investigators in the appropriate immunization of animals, collection of immune sera and provide information on possible antibody screening strategies to Project Scientists. Cell fusion and the subsequent cloning and cryopreservation of specific hybridomas will be performed by the Washington University School of Medicine Hybridoma Center. Once the desired hybridomas have been identified by the Project Scientists, the Antibody Production Core will generate antibody-containing supernatants, purify these monoclonal antibodies and provide to the Program Project Laboratories purified quantities of monoclonal antibody derived from ten liter cultures of hybridoma cells.