CORE--IMAGING FACILITY

成像核心的主要目标是促进定量和定性成像技术在细胞和分子水平上分析多囊肾疾病。适用于细胞、组织、整个器官以及野生型、转基因和突变动物研究的成像技术的范围得到了极大的扩展。新技术现在可用于更高分辨率的特定靶蛋白定位以及允许在其中可识别动态变化的活体显微术。这些方法包括免疫细胞化学、共聚焦显微镜、电子显微镜、原位杂交、无偏体视形态学和活体显微镜。这些技术在细胞生物学中非常有用，但不容易被没有受过训练或经验的研究人员所使用。 此外，选择适当的技术或技术组合需要解释经验和了解方法的局限性。成像核心将提供专业知识和援助，利用光，免疫荧光，共聚焦，和电子显微镜，特别强调转基因模型的表型和病理分析，免疫细胞化学，重要的显微镜，无偏见的体视形态学和图像分析。该设施将提供这些专业技术和应用这些技术所需的设备，并将成为该计划项目成员之间合作的焦点。具体而言，核心将提供必要的设施，服务和教育，以评估成像技术的应用，以适应该计划项目的各个成员的个人研究目标。

The major goal of the Imaging Core is to facilitate the use of quantitative and qualitative imaging techniques in the analysis of polycystic kidney disease the cellular and molecular level. The range of imaging techniques which are applicable to the study of cells, tissues, whole organs and in wild type, transgenic and mutant animals he been greatly expanded. new technologies are now available for greater resolution of localization of specific target proteins as well as permitting vital microscopy where dynamic changes can be identified. These include immunocytochemistry, confocal microscopy, electron microscopy, in situ hybridization, unbiased stereological morphometry, and vital microscopy. These techniques are extremely useful in cell biology but are not easily accessible to investigators without previous training or experience. In addition, the selection of the proper technique or combination of techniques requires experience in interpretation and knowledge of the limitations of the method. The Imaging Core will provide expertise and assistance in the utilization of light, immunofluorescence, confocal, and electro microscopy with particular emphasis on phenotypic and pathologic analysis of transgenic models, immunocytochemistry, vital microscopy, unbiased stereological morphometry and image analysis. The facility will give access to these specialized techniques and to the equipment necessary to apply them and will form a focal point for collaboration between members of the program project. Specifically, the Core will provide the facilities, service and education necessary for the evaluation application of imaging techniques tailored to the individual research objectives of the various members of the program project.