Core--Toxin probes and assays

毒素探测器和Assay Core将为这四个项目的每个项目提供分析支持。支持的形式包括：(1)毒素标准，(2)作为特定结合分析试剂的抗毒素抗体和突触体，(3)可能包含代谢物、结合物和光解产物的毒素衍生物，(4)傅立叶变换红外和核磁共振谱，(2)标准物质和合成代谢物或原产物的共振光谱分析，以及(5)高效液相色谱分析。对于研究项目1，核心预期支持项(1)、光解(3)和(5)是最重要的。对于研究项目2，支持项目(1)、(2)、(3)和(5)将是必不可少的。用于研究项目(3)可能包含代谢物、结合物和光解产物的毒素衍生物，(4)标准物质和合成代谢物或原产物的傅立叶变换红外和核磁(质子和碳)共振光谱分析，以及(5)高效液相色谱分析。对于研究项目1，核心预期支持项(1)、光解(3)和(5)是最重要的。对于研究项目2，支持项目(1)、(2)、(3)和(5)将是必不可少的。对于研究项目2、3，支助项目(1)和(2)将提供最大的好处。对于研究项目4，支持项目(1)和(2)将是最重要的。核心还将提供抗体和试剂毒素设施核心2，免疫细胞化学核心，以便免疫过氧化物酶分析可以进行与吞噬积累和生物标记物的发展。这与吞噬细胞蓄积和生物标记物检测的发展有关。后一项工作将需要合成抗体-HRP结合物，并测量每个结合单位的特定酶活性。为了有效地执行这一计划，巴登博士将监督S的核心职能，确定每个项目的努力程度，确定最常用的标准分析和衍生品，并与两名研究人员合作，及时提供材料和分析后勤。这两名研究助理将接收样本，定量分离和鉴定成分，开发合成方法以生产足够数量的受控实验，并就要收集的材料数量、保存方法和运输方法向个别项目提供建议。

The Toxin Probes and Assays Core will provide analytical support to each of the four projects. Support will be in the form of (1) toxin standards, (2) anti-toxin antibodies and synaptosomes as reagents for specific binding assays, (3) toxin derivatives that may encompass metabolites, conjugates, and photolysis products, (4) Fourier transform infrared and nuclear magnetic and proton and carbon) resonance spectrometry of standards and synthetic metabolites or proto-products, and (5) high performance liquid chromatographic analysis. For Research Project 1, the core anticipates support items (1), photolytic (3), and (5) to be the most important. For Research Project 2, support items (1), (2), (3) and (5) will be essential. For Research Project (3) toxin derivatives that may encompass metabolites, conjugates, and photolysis products, (4) Fourier transform infrared and nuclear magnetic (proton and carbon) resonance spectrometry of standards and synthetic metabolites or proto- products, and (5) high performance liquid chromatographic analysis. For Research Project 1, the core anticipates support items (1), photolytic (3), and (5) to be the most important. For Research Project 2, support items (1), (2) , (3) and (5) will be essential. For Research Project 2, 3, support items (1) and (2) will provide the most benefit. For Research Project 4, support items (1) and (2) will be the most important. The core will also provide antibodies and reagent toxins to Facilities Core 2, the Immunocytochemistry Core, so that immunoperoxidase assays an be carried out relative to phagocytic accumulation and biomarker assay development. This relative to phagocytic accumulation and biomarker assay development. This latter effort will require synthesis of antibody- HRP conjugates and measurement of specific enzymatic activity per binding unit. To carry out this Program efficiently, Dr. Baden will oversee the core s functions, determine the level of effort to be expended on each project, identify the standards assays, and derivatives that will be of most use, and work with the 2 research associates to provide the materials and assay logistics and a timely manner. The two research associates will receive samples, isolate quantitatively and identify the components, develop synthetic methods to produce sufficient quantities for controlled experiments, and provide advice to individual projects pertaining to amounts of material to collect, methods of preservation, and methods of transport.