VASCULAR PATTERNING IN LEAVES OF ARABIDOPSIS THALIANA

我将研究PROV1在拟南芥叶片维管模式形成中的作用。 叶片中的维管图案由位置线索指定，并且发育中的静脉本身可能充当叶片中细胞类型随后图案化的线索。 PROV1基因最近被鉴定，使用增强子陷阱策略，其在年轻的血管原细胞中特异性表达，克隆时被发现编码受体样蛋白激酶。 PROV1在维管特化和分化中的功能将通过在成熟植物中过表达、过表达和有条件表达PROV1来解决。 将评估激酶活性，通过原位免疫定位确定亚细胞定位，并使用酵母双杂交系统中的异源表达鉴定相互作用蛋白。 PROV1在静脉发育中的作用的表征和对调节静脉图案形成的机制的深入了解将导致在植物器官发生过程中理解位置规范的有价值的进展。

I will investigate the role of PROV1 in vascular pattern formation in leaves of Arabidopsis thaliana. Vascular patterning in leaves is specified by positional cues and the developing veins themselves likely act as cues for the subsequent patterning of cell types in the leaf. The gene PROV1 was recently identified, using an enhancer trap strategy, for its specific expression in young provascular cells and when cloned was found to encode a receptor-like-protein-kinase. The function of PROV1 in vascular specification and differentiation will be addressed by over-, under-, and conditionally expressing PROV1 in mature plants. The kinase activity will be evaluated, the subcellular localization will be determined by imunolocalization in situ, and interacting proteins will be identified using heterologous expression in the yeast two-hybrid system. The characterization of the role of PROV1 in vein development and insight into mechanisms which regulate vein pattern formation will result in valuable progress toward understanding positional specification during plant organogenesis.