CORE--TRANSGENIC MICE

描述：转基因小鼠核心的主要功能是 通过显微注射重组DNA到小鼠体内产生转基因小鼠 胚胎转基因小鼠越来越多地被用于研究 神经元发育和神经元特异性基因表达。实验 使用转基因小鼠的研究包括：a）顺式作用调节 引导基因表达至特定神经元细胞类型的序列; B） 研究细胞表面标记物的不适当合成， 正常神经元形态发生所必需的; c）神经元的研究 成熟、细胞周期控制和凋亡;以及d）轴突的研究 寻路这些研究有助于导致精神障碍的疾病， 迟钝 用于制备转基因小鼠的重组DNA构建体将 由用户实验室产生，但核心，直到将净化 用于显微注射的DNA片段。注射后，胚胎将 再植入假孕的养母体内发育至足月 在小鼠出生和断奶年龄后，核心将在耳上贴上标签， 小鼠进行识别，之后将动物转移至 个别研究者进行进一步表征。 核的另一个功能是低温保存 转基因小鼠品系对于这些实验，个体 研究者将向核心提供超数排卵的妊娠女性， 将收获单细胞期的小鼠胚胎并冷冻保存在 使用标准方法的人工授精吸管。的成功 通过从一根吸管中回收胚胎来监测冷冻保存 并检查胚胎发育到 囊胚期。

DESCRIPTION: The primary function of the Transgenic Mouse Core is to generate transgenic mice by microinjection of recombinant DNA into mouse embryo. Transgenic mice are increasingly being used for studies of neuronal development and neuron-specific gene expression. Experiments using transgenic mice include: a) studies of cis-acting regulatory sequences that direct gene expression to specific neuronal cell types; b) studies of inappropriate synthesis of cell surface markers that are essential for normal neuronal morphogenesis; c) studies of neuronal maturation, cell cycle control and apoptosis; and d) studies of axonal path-finding. Such studies contribute to disorders that can lead to mental retardation. The recombinant DNA constructs to be used for making transgenic mice will be generated by the user laboratories, but the core until will purify the DNA fragments for microinjection. After injection, the embryos will be reimplanted into pseudopregnant foster mothers for development to term. After the mice are born and of weaning age, the Core will ear tagged the mice for identification, after which the animals will be transferred to individual investigators for further characterization. Another function to be served by the core is the cryopreservation of transgenic mouse lines. For these experiments, the individual investigators will provide superovulated pregnant females to the Core, and mouse embryos at the one cell stage will be harvested and cryopreserved in artificial insemination straws using standard methods. The success of cryopreservation will be monitored by recovering embryos from one straw and examining the efficiency with which the embryos develop to the blastocyst stage in vitro.