HERMES AND MARINER TRANSFORMATION OF AEDES AEGYPTI

病媒传播的疾病继续造成大量死亡和 世界各地人口的发病率。而疟疾和黄色 热病是欠发达国家的祸害，是登革热的威胁 发烧正在美国边境蔓延。对传输的控制 这些疾病中的一种可以通过控制它们的昆虫媒介来实现。 蚊子转基因技术的发展使蚊子的生产成为可能。 已经被稳定插入的蚊子的 外源基因。我们的长期目标是培育出转基因蚊子 它们能抵抗病原体的感染，并将它们作为系统 用于研究病原体与病媒的相互作用以及释放生物体 寻求对寄生虫传播进行遗传控制的程序 和病毒性疾病。更热的，变形使之有可能 研究BASIC涉及的生化和分子生物学过程 鱼的生理方面，如血液喂养和消化， 卵子发生和寄主寻找。基于转座子的遗传分析 蚊子有可能识别出许多关键的基因，这些基因 表达对载体能力和载体能力具有重要意义。 最近，我们已经证明了TWSO第二类转座元件Hermes 和水手，调解外源DNA插入到染色体中 黄热病蚊子。埃及伊蚊(Jasinski ene等人，1998； Coates等人，1998)。这些转变的进一步完善 需要系统来促进它们的使用来产生病原体- 对蚊子和其他基本生物菌株产生抗药性 为此，我们提出了以下具体目标：1) 开发常规分离DNA片段的方法，包括 来自Hermes转化蚊子品系的转座子-染色体连接； 2)开发爱马仕的再动员程序和菌株 Mariner染色体插入；3)产生转基因菌株 利用cre-loxP和FLP-FRT位点特异性重组的蚊子 用于比较启动子分析。成功实现这些目标 具体目标将导致强大和广泛适用的系统或 蚊子转基因。

Vector-borne diseases continue to cause significant mortality and morbidity in people throughout the world. While malaria and yellow fever are scourges of lesser-developed countries, the threat of Dengue fever is at the borders of the United States. Control of transmission of these diseases can be achieved by controlling their insect vectors. The development of mosquito transgenesis has made possible the producion of mosquitoes that have been altered by the stable insertion of exogenous genes. Our long-term goal is to produce transgenic mosquitoes that are resistant to infection by pathogens and use these as systems for studying pathogen-vector interactions as well as release organisms for programs seeking genetic control over the transmission of parasitic and viral diseases. Furthermoe, transormation makes it possible to study biochemical and molecular biological processes involved in basic aspects of mosuito physiology such as blood feeding and digestion, oogenesis and host seeking. Transposon-based genetic analysis of mosquitoes has the potential to identify many crucial genes whose expression is important for vector competence and vectorial capacity. Recently, we have shown that twso Class II transposable elements, Hermes and mariner, mediate the insertion of exogenous DNA into the chromosomes of the yellow fever mosquito. Aedes egypti (Jasinskiene et al., 1998; Coates et al., 1998). Further refinements of these transformation systems are reuqired to facilitate their use in producing pathogen- resistant mosquitoes and other strains for basic biological study.Towards these ends we propose the following specific aims: 1) develop methods for routine isolation of DNA fragments consisting of transposon-chromosome junctions from Hermes-transformed mosquito lines; 2) develop procedures and strains for remobilization of Hermes and Mariner chromosomal insertions; 3) produce transgenic strains of mosquitoes that exploit cre-loxp and FLP-FRT site-specific recombination for comparative promoter analysis. Successful accomplishment of these Specific Aim will result in robust and widely-applicable systems or mosquito transgenesis.