Mosquito innate immune responses to arbovirus infections

蚊子对虫媒病毒感染的先天免疫反应

• 批准号: 7675036
• 负责人: Anthony A. James
• 金额: $ 21.22万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-05-01 至 2014-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7675036
• 关键词: Aedes; Arbovirus Infections; Arboviruses; Bacterial Infections; Code; Culicidae; Dengue; Development; Emerging Communicable Diseases; Epidemiology; Gene Amplification; Gene Expression; Genes; Genetic; Genetic Transcription; Genome; Goals; Health; Human; Immune response; Infection; Infectious Diseases Research; Knowledge; Methods; Molecular Profiling; Pattern; Proteins; Regulation; Reporter; Research; Role; Testing; Transgenes; Untranslated RNA; Viral; Viral Vector; Virus; Virus Diseases; Yellow Fever; base; biodefense; chikungunya; design; disorder control; in vivo; member; novel; pathogen; promoter; response; transmission process; vector; vector mosquito

The genes involved in mosquito immune responses to parasitic and bacterial infections have been studied extensively, however similar knowledge of vector responses to viruses is limited. Our long-term goal is to use knowledge of mosquito responses to viruses to design novel disease-control methods. This proposed research focuses on the development and usage of whole-genome tiling arrays for analyses of gene expression in the mosquito vector, Aedes aegypti, following infection with arboviruses. The information generated by this study will reveal global and specific responses of mosquitoes to a number of viral pathogens, and provide materials for the development of genetics-based disease control strategies. The Specific Aims are: i) to design whole-genome tiling arrays containing interrogation probes for the genomes of Ae. aegypti, and the arboviruses, Dengue, Yellow Fever and Chikungunya; 2) to identify mosquito protein-coding and noncoding RNAs with altered transcription patterns following infection with viruses and validate their expression profiles by quantitative gene amplification; and 3) to design transgenes expressing reporter molecules under the regulation of virus infection-induced mosquito promoters, and test in vivo the functionality of the control sequences.

参与蚊子对寄生虫和细菌感染的免疫反应的基因已经被发现。 虽然对这些病毒进行了广泛的研究，但关于病媒对病毒的反应的类似知识有限。我们的长期目标 是利用蚊子对病毒的反应来设计新的疾病控制方法。这一拟议 研究重点是开发和使用全基因组平铺阵列进行基因表达分析 在蚊子媒介埃及伊蚊感染虫媒病毒后。产生的信息 这项研究将揭示蚊子对一些病毒病原体的全球性和特异性反应，并提供 为制定基于遗传学的疾病控制策略提供材料。具体目标是：（一） 设计包含Ae基因组询问探针的全基因组平铺阵列。埃及和 虫媒病毒、登革热、黄热病和基孔肯雅热; 2）鉴定蚊子蛋白编码和非编码 病毒感染后转录模式改变的RNA及其表达验证 通过定量基因扩增来设计表达报告分子的转基因;以及3）设计表达报告分子的转基因， 调节病毒感染诱导的蚊子启动子，并在体内测试对照的功能 序列的