THE S. CEREVISIAE S PHASE CHECKPOINT

描述：（改编自研究者摘要）。该提案将使用 酵母模型系统，探索协调DNA完成的检查点 有丝分裂复制。具体而言，本提案将研究如何 酵母细胞感觉到复制还没有完成， 从中期到后期。这个检查站的重要性是显而易见的， 在细胞复制之前， 遗传物质是一个灾难性的事件，导致瞬时损失， 基因组完整性该提案的重点是几个保守的蛋白质， 通过基因分析确定，其中一些以前没有被发现。 与检查点功能相关。具体目标是描述 依赖于Pds 1延迟的“晚”S期检查点的参数 后期，研究Rad 23和Ddi 1在Pds 1依赖的S 阶段检查点，并进行详细的结构/功能分析， Rad 23和Ddi 1蛋白。

DESCRIPTION: (Adapted from the Investigator's Abstract). This proposal will use a yeast model system to explore a checkpoint that coordinates completion of DNA replication with mitosis. Specifically, this proposal will investigate how a yeast cell, sensing that replication is not yet complete, blocks the transition from metaphase to anaphase. The importance of this checkpoint is obvious, in that entry into the process of cell division prior to duplication of the genetic material is a catastrophic event, leading to instantaneous loss of genomic integrity. The proposal focuses on several conserved proteins that were identified through genetic analysis, some of which previously had not been associated with checkpoint functions. The specific aims are to characterize the parameters of a "late" S phase checkpoint that depends on Pds1 to delay anaphase, to investigate the role of Rad23 and Ddi1 in the Pds1-dependent S phase checkpoint and to engage in a detailed structure/function analysis of the Rad23 and Ddi1 proteins.