Effects of Sex and Estrogen on Drug Self-Administration

描述：(申请人提供) 这项建议旨在检验性和雌激素对 静脉自给药(静脉注射)在老鼠身上给药。四 动物模型将被用来研究获取、维护、 管制/失调，以及恢复药物自我管理。这个 性别和雌激素对海洛因、甲基苯丙胺和甲基安非他明的影响 D，1-3，4-亚甲基二氧基甲基苯丙胺(MDMA)自我给药 使用自动整形程序进行了检查。一种累进比率(PR)时间表 强化和对需求的行为经济分析将被用于 评估性和雌激素对可卡因和 海洛因自我给药。PR进度表上获得的断点(BP)措施； 与从需求曲线获得的斜率和P-max值一起，将用于 评估几种剂量的可卡因和海洛因的增强效果。 将利用差别获得可卡因自我管理的机会来调查 性别对可卡因摄入量调节/失调的影响 将获得可卡因的剂量-反应曲线，以检查组内差异 剂量-反应曲线的变化。雌激素对子宫内膜异位基因表达的影响 将使用引爆剂检查恢复可卡因的自我给药 毒品恢复模型，其中杠杆按压毒品被熄灭 用生理盐水代替可卡因的输注。恢复答辩 然后通过注射可卡因进行测试。

DESCRIPTION: (provided by applicant) This proposal was designed to examine the effects of sex and estrogen on the self-administration of intravenously (i.v.) delivered drugs in rats. Four animal models will be used to investigate acquisition, maintenance, regulation/dysregulation, and reinstatement of drug self-administration. The effects of sex and estrogen on the acquisition of heroin, methamphetamine and d, 1-3, 4-methylenedioxymethamphetamine (MDMA) self-administration will be examined using an autoshaping procedure. A progressive ratio (PR) schedule of reinforcement and a behavioral economic analysis of demand will be used to evaluate the effects of sex and estrogen on the maintenance of cocaine and heroin self-administration. Breakpoint (BP) measures obtained on a PR schedule, along with slopes and P-max values obtained from demand curves will be used to evaluate the reinforcing efficacy of several doses of cocaine and heroin. Differential access to cocaine self-administration will be used to investigate the effect of sex on the regulation/dysregulation of cocaine intake. Dose-response curves for cocaine will be obtained to examine group differences in shifts in the dose-response curves. The effect of estrogen on the reinstatement of cocaine self-administration will be examined using a priming model of drug reinstatement in which lever pressing for drug is extinguished by replacing cocaine infusions with saline infusions. Reinstatement of responding is then tested by administering a priming injection of cocaine.