CYTOPLASMIC MRNA TRANSPORT IN XENOPUS OOCYTES

描述：本提案的目标是了解 管理Vg1 RNA在非洲爪哇卵母细胞上的定位。以前的实验 在施纳普的实验室中已经表明，这种本地化需要 3‘非编码区中存在的四个不同重复序列中的每一个至少一个拷贝 MRNA.5个核苷酸重复序列UUCAC(E2)似乎发挥了重要作用 是这笔赠款的大部分重点。施纳普发现了一种蛋白质，维拉， 绑定到VgLE，很可能是通过与 重复E2。因为Vera是一种内质网相关蛋白，Schnapp提出 它在将Vg1RNA定位到内质网中发挥作用。 Schnapp还成功分离出一种含有Vg1的RNP颗粒 本地化元素(VgLE)。所有四种VgLE重复类型都需要 VgLE在粒子中的关联。这与早先的情况形成了鲜明对比 观察到只需要三个(E1、E2、E4)即可与 Vera蛋白，这反过来表明后者不足以 粒子集合。

DESCRIPTION: The goal of this proposal is to understand the mechanisms that govern localization of Vg1 RNA on the Xenopus oocyte. Previous experiments in Schnapp's lab have shown that this localization requires the presence of at least one copy each of four different repeats present in the 3'UTR of the mRNA. A five nucleotide repeat UUCAC (E2) appears to play a major role and is the focus of much of this grant. Schnapp has identified a protein, vera, that binds to the VgLE, most likely by direct physical association with the E2 repeat. Because Vera is an ER associated protein, Schnapp proposes that it plays a role in localizing Vg1RNA to the ER. Schnapp has also succeeded in isolating a RNP particle containing the Vg1 localization element (VgLE). All four VgLE repeat types are required for the VgLE's association in the particle. This contrasts with the earlier observation that only three (E1,E2,E4) are required for association with vera protein, suggesting in turn that the latter is not sufficient for particle assembly.