COCHLEAR BLOOD FLOW AND NEUROPEPTIDES

偏头痛相关的内耳症状包括恐音、耳鸣、听力波动、听力损失和噪声敏感性增加，这为基底动脉偏头痛和耳蜗病理生理机制之间可能存在神经基础提供了证据。最近，我们发现了一个以前未报道的耳蜗血管的感觉神经支配起源于三叉神经节。我们已经证明，这种感觉神经支配在正常和病理条件下（例如，在梅尼埃病症状之一的内淋巴水肿动物模型中）都对耳蜗血流量（CBF）有显著影响。本研究旨在进一步确定椎体血管和耳蜗血管系统周围三叉神经感觉网络的解剖学基础和机制。提出三叉神经感觉系统及其相关神经肽系统是影响颅底偏头痛和耳蜗血管稳态的重要因素。这项研究有三个具体目的。目的1。目的：探讨基底动脉偏头痛患者耳蜗症状是否存在生理基础。阳性结果将证实颅底偏头痛和耳蜗症状的共同功能基础，其基础可能是神经源性炎症。目标2。目的探讨香草样受体（VR1）和P物质（SP）是否在耳蜗血管、基底动脉及其相关分支周围共定位。阳性的免疫细胞化学结果将显示：(a) VR1和(b) SP共同标记的初级感觉神经元在基底动脉周围的网络；小脑前下动脉（AICA）、螺旋磨牙动脉（SMA）和桡动脉；(c)辣椒素会导致标记的感觉纤维密度显著降低。目标3。目的：探讨内淋巴水肿时三叉感觉神经元的血管调节紊乱。在这项研究中，阳性结果将表明，内淋巴水肿导致刺激的三叉神经节诱导的CBF变化减少。该建议的研究将有助于阐明三叉感觉神经元在正常和病理条件下如何调节椎基底血管系统和耳蜗液平衡。

The migraine related inner ear symptoms for phonopobia , tinnitus, hearing fluctuation, hearing loss, and increased noise sensitivity provide evidence for a possible neurological substrate connecting basilar artery migraine and cochlear pathophysiological mechanisms. Recently we have identified a previously unreported sensory innervation of the cochlear blood vessels originating from the trigeminal ganglia. We have shown that this sensory innervation has a significant effect on cochlear blood flow (CBF) in both normal and pathological conditions (e.g., in the animal model of endolymphatic hydrops, one of the symptoms of Meniere's disease). This proposal seeks to further define the anatomical basis and mechanisms of the trigemino-sensory network around the vertebrovasilar and cochlear vascular system. The proposal offers the hypothesis that the trigemino-sensory system and its related neuropeptide system are important factors contributing to basilar migraine and vascular homeostasis of the cochlea. The study has three specific aims. Aim 1. To establish if there is a physiological basis for the cochlear symptoms in basilar artery migraine headache. Positive results will confirm a common functional basis for basilar migraine and cochlear symptoms, the basis could be neurogenic inflammation. Aim 2. To demonstrate if vanilloid receptor (VR1) and substance P (SP) are co-localized around cochlear blood vessels, the basilar artery and its related branches. Positive immunocytochemical results will demonstrate: (a) network of the VR1 and (b) SP co-labeled primary sensory neurons around the basilar artery; anterior inferior cerebellar artery (AICA), spiral modiolar artery (SMA) and radial artery; (c), Capsaicin will cause a significant reduction in the density of labeled sensory fibers. Aim 3. To determine the vasoregulatory disturbance of the trigemino-sensory neurons in endolymphatic hydrops. In this study positive results will demonstrate that endolymphatic hydrops causes a reduction in the stimulated trigeminal ganglion induced CBF change. The studies of the proposal will help clarify how trigemino-sensory neurons regulate the vertebro-basilar vascular system and cochlear fluid balance under normal and pathological conditions.