Proteome Markers for Alzheimer's Disease Diagnosis

描述(摘自申请者的摘要)：本项目的目标 是确定与生前诊断相关的脑脊液分子标志物 阿尔茨海默氏症可以作为疾病病理学的一种衡量标准。在……里面 与基于大脑的疾病分子机制研究形成对比 进展，这项研究利用蛋白质组学技术来鉴定和 鉴定感兴趣的脑脊液蛋白。此信息可用于 对疾病病理进行分类，或可用于开发免疫分析方法 诊断。为了实现这些长期目标，调查员 计划：识别和获取脑脊液血液样本和临床信息 不同形式的痴呆症患者的适当样本人群， 不同阶段可能的阿尔茨海默病和对照(具体目标1)。 分析样品中用于蛋白质组分析的AD特异性蛋白质标志物 这包括高溶液双向电泳、激光 密度测量、荧光成像和计算机辅助的每种分析 (具体目标2)。他使用多元统计方法来建立相关性 在疾病诊断的相关变化和建立定性和 用于诊断阿尔茨海默病的定量条形码。这包括 验证其他建议的脑脊液标志物，如tau和Abeta42以及 其他主要鉴别诊断的初步鉴定 血管性痴呆、路易体痴呆(具体目标3)。使用 重要蛋白质的显微化学特征用于阐明遗传 分子标记和其他参考蛋白质的基础。此信息 也可用于开发免疫分析(特定目标4)。

DESCRIPTION (Adapted from applicant's abstract): The objective of this project is to identify CSF molecular markers associated with the antemortem diagnosis of Alzheimer's disease which can be used as a measure for disease pathology. In contrast to brain based studies of the molecular mechanism of disease progression, this investigation with use proteomics technology to identify and characterize CSF proteins of interest. This information can be used to categorize disease pathology or can be used to develop immunoassays for diagnosis. In order to achieve these long-term objectives, the investigator plans to: identify and obtain CSF blood samples and clinical information from an appropriate sample population of patients with different forms of dementia, probable Alzheimer's disease at various stages and controls (Specific Aim 1). Analyze the samples for AD specific protein markers used in proteome analysis that includes high solution two-dimensional electrophoresis, laser densitometry, fluorescence imaging, and computer aided in each analysis (Specific Aim 2). He employs multivariate statistics to establish a correlation between relevant changes in disease diagnosis and establish qualitative and quantitative bar code for diagnosis of Alzheimer's disease. This includes the validation of other proposed CSF markers such as tau and Abeta 42 as well as the preliminary identification of other major differential diagnosis such as vascular dementia, dementia with Lewy bodies (Specific Aim 3). Use microchemical characterization of important proteins to elucidate the genetic basis of the molecular markers and other reference proteins. This information can also be used to develop immunoassays (Specific Aim 4).