Tenascin in cerebrospinal fluid is a useful biomarker for the diagosis of brain tumor

脑脊液中的腱蛋白是诊断脑肿瘤的有用生物标志物

• 批准号: 06671383
• 负责人: WAKABAYASHI Toshihiko
• 金额: $ 1.41万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671383/
• 关键词: Tenascin; Brain tumor; Extracellular matrix; Glioma; Tumor marker; Monoclonal antibody; CSF; Diagnosis

Tenascin, an extracellular matrix glycoprotein, has been reported to be expressed predominantly on glioma tissue in the central nervous system, both in a cell associated and an excreted form. Recently, a highly sensitive sandwich type enzyme immunoassay for quantitative determination of tenascin was developed. In the present study, the amount of tenascin in cerebrospinal fluid was measured. An increase of tenascin in CSF (>100 ng/ml) was found in patients with an astrocytic tumor. The concentration was significantly higher (>300ng/ml) in high grade astrocytoma (anaplastic astrocytoma and glioblastoma) and a further increase (>1000 ng/ml) was found in cases of CSF dissemination of high grade astrocytoma. On the other hand, tenascin concentrations were less than 100 ng/ml in non-astrocytic tumors and non-neoplastic neurological diseases, expect meningeal dissemination of tumor cells, meningeal stimulation by infection, and subarachnoid hemorrhage. In cases of treated astrocytoma in remission, tenascin was negligble (<100 ng/ml) in the CSF.The measurement of tenascin in CSF is useful for differential diagnosis of brain tumors and monitoring of astrocytic tumors.

Tenascin是一种细胞外基质糖蛋白，据报道主要在中枢神经系统的胶质瘤组织中表达，既以细胞相关形式表达，也以分泌形式表达。最近，建立了一种高灵敏度的三明治型酶免疫分析法来定量测定腱黄素。本研究测定了脑脊液中腱黄素的含量。星形细胞瘤患者脑脊液中腱素升高（bbb100 ng/ml）。高级别星形细胞瘤（间变性星形细胞瘤和胶质母细胞瘤）的浓度明显升高（>300ng/ml），脑脊液播散性星形细胞瘤的浓度进一步升高（>1000 ng/ml）。另一方面，在非星形细胞性肿瘤和非肿瘤性神经系统疾病中，除了肿瘤细胞的脑膜播散、感染引起的脑膜刺激和蛛网膜下腔出血外，tenascin浓度低于100 ng/ml。在治疗后缓解的星形细胞瘤病例中，脑脊液中的tenascin可忽略不计（<100 ng/ml）。脑脊液tenascin的测定对脑肿瘤的鉴别诊断和星形细胞肿瘤的监测有重要意义。

项目成果

吉田 純: "ヒトグリオーマ細胞に高発現するCD44とテネイシンの解析" 新村眞人, 3 (1994)

Jun Yoshida：“人神经胶质瘤细胞中高表达的 CD44 和腱生蛋白的分析”Masato Niimura，3 (1994)

J Yoshida, T Wakabayashi, S Kimura, K Washizu, K Kiyosawa, K Mokuno: "Tenascin in cerebrospinal fluid is an useful biomarker for the diagnosis of brain tumor." J Neurol Neurosuirg Psychi. 57. 1212-1215 (1994)

J Yoshida、T Wakabayashi、S Kimura、K Washizu、K Kiyosawa、K Mokuno：“脑脊液中的腱蛋白是诊断脑肿瘤的有用生物标志物。”

J Yoshida: "Tenascin in cerebrospinal fluid is a useful biomarker for the diagnosis of brain tumour" Journal of Neurology,Neurosurgery,and Psychiatry. 57. 1212-1215 (1994)

J Yoshida：“脑脊液中的腱蛋白是诊断脑肿瘤的有用生物标志物”《神经病学、神经外科和精神病学杂志》。