CREATING A DNA MICROARRAY AND CDNA SPOTTING FACILITY

描述：(申请人的描述)医学院建议 建立DNA微阵列和cDNA点杂交设施，以增强 国家肿瘤研究所立项资助和新开发项目 石溪大学的癌症研究人员。应用程序在很大程度上寻求支持 获得交钥匙DNA微阵列系统和单独的 扫描仪支持现有的cdna斑点能力。强大的机构 为成功申请提供支持，大约2.5-fo1d 一项奖项所提供的支持。该机构有一条经过验证的轨道 在创建和维护核心设施方面的记录，并将承担 负责建立和管理DNA微阵列的核心设施 和c DNA斑点分析。由国家癌症研究所资助的既定项目 研究所包括：受RAS和RAS调控基因的表达谱 与肿瘤发生有关；转录的特征 由VaV家族的两个成员诱导的激活/失活 致癌基因.编码分子的特定基因表达的分析 与癌进展中的表面蛋白相关；微阵列 表征关键细胞周期调控转录因子的作用 在酵母中；分析Run结构域蛋白的转录靶标； 白血病多发性骨髓瘤中FGFR3调控基因的表达分析 细胞系；E2F复合体表达调控基因的鉴定 含有病毒或细胞调节蛋白的；基因鉴定 受p73基因各种异构体的表达调控；以及 生长调节荷尔蒙干扰素调控的基因图谱， 催乳素和白介素4。计算问题方面的专家 微阵列将与癌症生物学家合作解决 生物信息学问题对这些技术的最佳利用至关重要。这个 将创建核心咨询委员会(由申请人担任主席)并由其组成 由用户提供监督和管理技术操作的问题， 根据需要，在未来对设施进行接入和升级。

DESCRIPTION: (Applicant's Description) The School of Medicine proposes to establish a DNA microarray and cDNA spotting facility to enhance the established programs of National Cancer lnstitute funded and newly-developing cancer researchers at Stony Brook. The application seeks support largely towards the acquisition of a turn-key DNA microarray system and a separate scanner to support an existing cDNA spotting capability. Strong institutional support is provided towards a successful application, approximately 2.5-fo1d over the support provided by an award. The institution has a proven track record in creating and maintaining core facilities and will assume the responsibility to establish and to manage a core facility in DNA microarray and cDNA spotting. The established programs funded by the National Cancer Institute include: expression profiling of genes that are regulated by Ras and are relevant to oncogenesis; characterization of transcriptional activation/deactivation induced by two members of the vav family of proto- oncogenes; analysis of the expression of specific genes encoding molecules associated with surface proteases in carcinoma progression; microarraying to characterize the role of critical cell cycle regulated transcription factors in yeast; analysis of transcriptional targets of the Runt domain proteins; analysis of gene expression regulated by FGFR3 in leukemia multiple myeloma cell lines; identification of genes regulated by expression of E2f complexes containing viral or cellular regulatory proteins; identification of genes regulated by the expression of the various isoforms of the p73 gene; and profiling of the genes regulated by the growth regulatory hormones interferon, prolactin, and interleukin-4. Experts in computational problems of microarraying will work in concert with the cancer biologists to address bioinformatic issues central to the optimal use of these technologies. The Core Advisory Board will be created (chaired by the applicant) and populated by users to provide oversight and to manage issues of technical operation, access, and upgrade of the facility in the future, as needed.