CAPILLARY ELECTROPHORESIS-MASS SPECTROMETER (CE-MS)

这是在国家研究资源中心共享仪器资助机制下对毛细管电泳质谱(CE-MS)仪器的请求。CE-MS系统将主要由斯克里普斯研究所(TSRI)的四名研究人员(Weiss、Henriksen、Gruol和Parsons博士)和加州大学圣地亚哥分校退伍军人管理局医学中心的一名研究人员(Purdy博士)组成的主要用户群体使用。主要用户群体中的每个研究人员都将使用CE-MS仪器来推进目前由NIH奖项资助的研究。CE-MS的几个特点将加速和推进主要用户群体的研究。首先，CE具有分离多种分析物的能力，如类固醇、大小肽、寡核苷酸、RNA转录本和DNA片段。其次，CE提供了无与伦比的拆分能力，并将提供我们研究中目前无法获得的重要分析物的选择性分离。质谱仪(MS)与毛细管电泳联用为使用这种分析技术提供了第三个优势。将MS提供的质量/电荷(m/z)信息和灵敏度与CE的分离特性相结合，在生物分子的明确识别和定量方面取得了巨大的进步。此外，MS扫描光谱还可用于新物质的结构鉴定。最后，CE-MS分析的样品要求非常少，这将大大减少对被监测生理系统的干扰，并将增加样品分析物浓度，提高我们测量的时间分辨率。CE-MS的这些特点将以目前不可能的方式推动当地科学界的研究努力。CE-MS将设在TSRI的“核心设施”实验室空间，内部咨询委员会将确保主要用户群体内外的研究人员使用和公平分享CE-MS。该委员会由首席调查员(帕森斯博士)、TSRI质谱学设施主任(Siuzdak博士)、具有丰富CE经验的高级研究员(Rivier博士)以及TSRI精神药理学和酒精研究中心主任(Koob博士)组成，每个主要用户都隶属于这两个组织。TSRI高级副总裁支持这一申请，并同意TSRI将承担CE-MS的长期运营和维护的财务责任。

This is a request for a Capillary Electrophoresis-Mass Spectrometry (CE- MS) instrument under the Shared Instrumentation Grant mechanism of The National Center for Research Resources. The CE-MS system will be utilized primarily by a major user group comprised of four investigators at The Scripps Research Institute (TSRI) (Drs. Weiss, Henriksen, Gruol and Parsons) and one investigator at the Veterans Administration Medical Center on the campus of UCSD (Dr. Purdy). Each of the investigators in the major user group will use the CE-MS instrument to advance research that is currently funded by NIH awards. There are several characteristics of CE-MS that will accelerate and advance the research of the major user group. First, CE has the ability to separate a wide variety of analytes such as steroids, large and small peptides, oligonucleotides, RNA transcripts and DNA fragments. Second, CE offers unparalleled resolution capabilities and will provide the separation of important analytes with a selectivity that is currently unavailable in our research. The coupling of mass spectrometry (MS) with CE presents a third advantage for using this analytical technique. The combination of the mass/charge (m/z) information and sensitivity provided by MS with the separation characteristics of CE provides a tremendous advance in the unambiguous identification and quantification of biological molecules. Moreover, MS scan spectra can be used for the structural elucidation of novel substances. Finally, the very small sample requirements for CE- MS analyses will enable a substantial reduction in the disturbance made to the physiological systems being monitored, and will increase sample analyte concentrations and improve the time resolution of our measures. These features of CE-MS will advance the research endeavors of the local scientific community in ways that are currently impossible. The CE-MS will be housed in 'core facility' laboratory space at TSRI, and an Internal Advisory Committee will assure the use and equitable sharing of the CE-MS among investigators both within and outside the major user group. This committee is comprised of the Principle Investigator (Dr. Parsons), the Director of the Mass Spectrometry Facility at TSRI (Dr. Siuzdak), a senior researcher with significant CE experience (Dr. Rivier) and the Director of both the Division of Psychopharmacology and the Alcohol Research Center at TSRI (Dr. Koob), organizations with which each of the major users are affiliated. The Senior Vice President of TSRI supports this application, and had agreed that TSRI will assume financial responsibility for the long-term operation and maintenance of the CE- MS.