NANO ESI ION TRAP MASS SPEC FOR PROTEOMINCS & MS(N)

13名研究人员正在申请资金，用于配备电喷雾、纳米电喷雾和APCI（大气压化学电离）离子源的离子阱质谱仪（MS）。离子阱质谱是奥尔巴尼研究界所没有的，将用于高灵敏度LC-MS/MS、肽序列、蛋白质组学和多阶段碰撞诱导解离[MS(n)]分析。这些研究人员由10个NIH和3个NSF资助，他们将直接受益于离子阱质谱的使用。其中7名研究人员将使用高灵敏度的质谱/质谱接口进行自动数据库搜索，以快速识别肽/蛋白质序列，即蛋白质组学。具体研究领域包括：金属诱导的免疫病理学、结核分枝杆菌与巨噬细胞的相互作用、线粒体在细胞凋亡中的作用、蛋白跨膜易位、血管生物学、古古菌中的应激基因调控、有丝分裂相关蛋白和PCB在人乳腺细胞中的雌激素效应。6名研究人员将使用质谱(N)分析来明确分子结构；不寻常的寡糖，肽二硫桥配配，广泛交联肽/蛋白质从海洋生物粘合剂，代谢物分析，复杂的有机地下水污染物，和辐射诱导的DNA修饰。所有共同研究者目前都与沃兹沃斯中心的生物质谱中心合作。首席研究员（核心主任）在生物质谱和多仪器、多用户设施的管理方面拥有丰富的经验。离子阱质谱将被集成到这个核心中。Wadsworth中心拥有大奥尔巴尼地区唯一的生物MS核心设施，并与奥尔巴尼医学院、纽约州立大学奥尔巴尼分校和伦斯勒理工学院的研究人员建立了常规分析和研究合作记录。该仪器将为研究人员提供自动化MS/MS蛋白质组数据库检索和MS(n)分析的唯一本地访问。此时，我们要求Finnigan LCQ Deca，具有Seaquest数据库搜索程序，并配备Protana纳米电喷雾离子源，作为最适合这些不同应用的仪器。

Thirteen investigators are requesting funds for an ion trap mass spectrometer (MS) equipped with electro spray, nanoelectrospray, and APCI (atmospheric pressure chemical ionization) ion sources. The ion trap MS, which is not available in the Albany research community, will be used for high sensitivity LC-MS/MS, peptide sequence, proteomics and multiple stage collision induced dissociation [MS(n)] analysis. These investigators are funded by 10 NIH and 3 NSF grants that will benefit directly from use of an ion trap MS. Seven of the investigators will use high sensitivity MS/MS interfaced to automated database searches for rapid identification of peptide/protein sequence, i.e. proteomics. Specific research areas include: metal-induced immunopathology, M. tuberculosis interaction with macrophages, the role of mitochondria in apoptosis, protein translocation across membranes, vascular biology, stress gene regulation in archea, mitosis associated proteins, and PCB estrogenic effects in human breast cells. Six of the investigators will use MS (N) analysis for molecular structure elucidation, specifically; unusual oligosaccharides, peptide disulfide bridge assignment, extensively cross- linked peptides/proteins from a marine organism adhesive, metabolite analysis, complex organic ground water pollutants, and radiation-induced DNA modifications. All co-investigators collaborate currently with the Biological Mass Spectrometry Core at the Wadsworth Center. The Principal Investigator (Director of the Core) has extensive experience in biological mass spectrometry and the management of a multi-instrument, multi-user facility. The ion trap MS will be integrated into this core. The Wadsworth Center houses the only biological MS core facility in the greater Albany area, and it has an established record of both routine analysis and research collaboration with investigators at Albany Medical College, SUNY Albany, and Rensselaer Polytechnic Institute. Award of this instrument will afford the only local access of automated MS/MS proteomic data base searching and MS(n) analysis for the investigators. At this time we request a Finnigan LCQ Deca, with the Seaquest database search program, and equipped with a Protana nanoelectrospray ion source, as the instrument best suited to these diverse applications.