STORM 860 PHOSPHORIMAGER

本申请资金购买磷光成像代表了一个协调计划的一部分，以建立一个上级基因治疗设施在威尔医学院的康奈尔大学。这个共享设施将为康奈尔大学和附属教师提供从基础研究到临床试验的所有基因治疗技术。为了促进高质量的基础研究，分子核心负责提供分析方法和仪器，以便对生物样品进行详细的分子分析。基因治疗的成功关键在于对基因转移效率的客观定量评估和对治疗组织状态的分析。在分子生物学中，X射线和照相胶片的传统使用限制了定量分析。这里要求的多功能磷光成像仪将使几种定量方法，使用荧光，化学荧光和放射性方法的分子数据分析。一个由12名研究人员组成的小组得到了广泛的研究支持，主要来自美国国立卫生研究院，他们被确定为基因治疗设施的最初用户，并代表了该设备的用户。预期的应用包括组织中治疗性和内源性基因表达的定量测定，以及使用基因阵列对基因表达和基因治疗效果进行全面调查。在基因治疗设施的总体组织结构和设施中，提供了地点和永久管理和监督机制，以确保最佳使用该仪器。

This application for funds to purchase a phosphorimager represents part of a coordinated plan to build a superior Gene Therapy Facility at The Weill Medical College of Cornell University. This shared facility will provide Cornell and affiliated faculty with access to all the technology to perform gene therapy from basic research to clinical trials. To promote high quality basic research, the molecular core is charged with providing analytical methods and instruments that allow detailed molecular analyses of biological samples. Success in gene therapy is critically dependent on objective quantitative assessment of the efficiency of gene transfer and analysis of the status of treated of tissues. The traditional use of X-ray and photographic film in molecular biology has limited quantitative analyses. The multi-functional phosphorimager requested here will enable several quantitative approaches to the analysis of molecular data using fluorescent, chemifluorescent and radioactive approaches. A group of twelve investigators with extensive research support, primarily from NIH sources, have been identified as the initial users of the Gene Therapy Facility and represent the users of the equipment. Among the intended applications are quantitative assays of therapeutic and endogenous gene expression in tissues and use of gene arrays to give comprehensive surveys of gene expression and the effect of gene therapy. In the overall organizational structure and facilities of the Gene Therapy Facility provide the location and permanent management and oversight mechanisms that ensures the optimal use of the instrument.