ACQUISITION OF A QUADRUPOLE ION TRAP-HPLC

ESI离子陷阱-高效液相色谱结合了电喷雾电离(ESI)质谱仪相当高的选择性和灵敏度以及精细的分析分离，可以服务于有机和生物有机化学家的研究目的。加州大学戴维斯分校化学系的四名被点名的研究员管理着包括分析、合成有机化学和生物有机化学的研究，这些研究严重依赖于获得MS。近年来，个别教员研究项目的发展带来了对分析方法的需求，其速度超过了现有的仪器。为了满足加州大学戴维斯分校化学系四个主要用户和各种次要用户的需求，我们需要一台高效液相-电喷雾质谱计，它将适应一系列涉及小分子(MW&lt；2,000)到小蛋白质(MW&lt；20,000)的研究实验，并提供与分析层析一致的软电离技术的新功能。每个主要用户的关键需求和共同研究主题是需要对依赖时间的现象进行实时MS测量(动力学、责任研究或对天然产品的分离和分离进行分析，这些不能通过提交常规MS服务有效地满足)。这四个主要用户总共管理着6个主要的NIH RO1或PO1拨款。Carlito Lebrilla用电喷雾电喷雾质谱(ESI MS/MS)研究了硫化和非硫化支链低聚糖的结构。克劳德·米尔斯描述了与金属螯合基团化学偶联的多肽的结构。支持三个天然产物化学项目。Tadeusz F.Molinski研究了海洋无脊椎动物生物活性小分子组分的分离和结构鉴定，以及钙通道激动剂Bastadin-5与RyR1通道的共同结合部位。沈本研究了模块化聚酮生物合成的分子遗传学、酶学和时间方面的问题。分配给四个主要用户的拟议仪器时间比例将为80%，其余时间分配给化学系内已确定的次要用户。

ESI ion trap-HPLC combines the considerable selectivity and sensitivity of electrospray ionization (ESI) mass spectrometry with fine analytical separations that can serve the research purposes of organic and bioorganic chemists. The four named investigators at the Department of Chemistry at UC Davis manage research that encompasses analytical, synthetic organic and bioorganic chemistry which are critically dependent upon access to MS. In recent years, the evolution of individual faculty research programs has brought demands for analytical methods that have outpaced available instrumentation. In order to meet the needs of four major users and various minor users in the Department of Chemistry, UC Davis, we request an HPLC -ESI ion trap mass spectrometer which will accommodate a range of research experiments involving small molecules (MW <2,000) to small proteins (MW<20,000) and provide new capabilities in soft ionization techniques in line with analytical chromatography. The critical need and common research theme for each major user is a need for real-time MS measurements of time-dependent phenomena (kinetics, liability studies, or analysis of separation & isolation of natural products that cannot be effectively accommodated by submission to routine MS services. The four major users manage a total of 6 major NIH RO1 or PO1 grants. Carlito Lebrilla studies the structures of sulfated and non-sulfated branched oligosaccharides using ESI MS/MS spectrometry. Claude Meares characterizes the structure of peptides chemically conjugated to metal chelating groups. Three natural products chemistry programs will be supported. Tadeusz F. Molinski studies the isolation and structure elucidation of biologically active small molecule components of marine invertebrates and the consensus binding site of the Ca2+ channel agonist, bastadin-5 with the RyR1 channel. Ben Shen investigates the molecular genetics, enzymology and temporal aspects of modular polyketide biosynthesis. The proportion of proposed instrument time allocated to the four major users will be 80% with the balance of the time made available to identified minor users within the department of chemistry.