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Acquisition of a High Resolution TOF Mass Spectrometer

购置高分辨率 TOF 质谱仪

基本信息

批准号：
7795051
负责人：
Tadeusz F Molinski
金额：
$ 30.52万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN DIEGO
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-04-22 至 2011-04-21
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The proposal seeks acquisition of a TOF mass spectrometer coupled with an HPLC for accurate mass determination of small molecules such as natural products, organometallic compounds, nucleic acids and non-polar organic synthetic intermediates that are generated in the research groups of six major NIH-supported research groups at three departments of University of California, San Diego. The HPLC-TOF will be used for determination of composition and formulas of novel natural products obtained from marine bacteria and invertebrates, characterize enzyme-catalyzed reactions of genetically-engineered polyketide synthase complexes, nucleic acids prepared with modified base and sugar components, organometallic compounds for novel carbon-carbon bond formation or for transformations of simple thermodynamically stable molecules such as CO2 and NO2. The separations capability of the LC component will allow measurements of very minor components of moderately complex fractionated natural product extracts, and 'real-time' monitoring of reaction pathways and intermediates in the biosynthesis of natural products. The instrumental specifications have been chosen to provide ionization of a broad range of sample types, from nonpolar organic intermediates to highly-charged water-soluble molecules of molecular masses up to ~2000 and small polypeptides of larger MW yet be amenable to walk-up usage in a multi-user environment.) PUBLIC HEALTH RELEVANCE: Research on drug molecules is highly dependent upon high-resolution mass spectrometry for accurate measurement of the masses of molecules. The proposed instrument will expedite research in discovery of new therapies for cancer and infectious from marine organisms and development of diagnostics by a collective group over 60 associated researchers.

描述（由申请人提供）：该申请寻求获得一台TOF质谱联用高效液相色谱（HPLC），用于精确测定天然产物、有机金属化合物、核酸和非极性有机合成中间体等小分子的质量，这些小分子是由美国国立卫生研究院支持的六个主要研究小组在加州大学圣地亚哥分校三个系的研究小组中产生的。HPLC-TOF将用于测定从海洋细菌和无脊椎动物中获得的新型天然产物的组成和配方，表征基因工程聚酮合成酶复合物的酶催化反应，用修饰的碱和糖组分制备的核酸，用于新型碳-碳键形成的有机金属化合物或用于简单的热力学稳定分子（如CO2和NO2）的转化。LC组分的分离能力将允许测量中等复杂的分离天然产物提取物的非常微小的成分，并“实时”监测天然产物生物合成中的反应途径和中间体。仪器规格已被选择，以提供广泛的样品类型的电离，从非极性有机中间体到高电荷水溶性分子的分子质量高达~2000和小多肽的较大MW，但可适应在多用户环境中步行使用。