Strand Invasion of the Collagen Triple Helix

描述（由申请人提供）：单链DNA可以侵入现有的 DNA双链体通过置换一条亲本链形成D环， 沃森-克里克碱基对。胶原蛋白中的类似过程 三螺旋代表了治疗皮肤创伤的另一种方法， 但是没有被观察到。单链胶原肽侵入 伤口两侧磨损的胶原原纤维实际上会闭合， 伤口不需要缝合。为了开发这种方法， 评估单链肽侵入胶原三螺旋的能力。 以半胱氨酸结为模板的异源三聚体三螺旋胶原模拟物 将被构建并添加到单链肽的溶液中 由已知形成超稳定三螺旋的三肽重复组成。 将通过荧光法测定单链与宿主的结合 各向异性和超离心。为了扩大单一的有效范围， 多个单链肽将被连接到伤口上， PAMAM树枝状聚合物的臂，以及这种多聚体结构 将测定侵入多个胶原宿主。

DESCRIPTION (provided by applicant): Single-stranded DNA can invade an existing DNA duplex to form a D-loop by displacing one parental strand and forming Watson-Crick base pairs to the other. The analogous process in the collagen triple helix represents an alternate method for the treatment of skin wounds, but it has not been observed. Single-stranded collagen peptides that invaded frayed collagen fibrils on opposite sides of a wound would, in effect, close the wound without the need for suturing. To develop this method, the ability of single-stranded peptides to invade the collagen triple helix will be assessed. A heterotrimeric, triple-helical collagen mimic templated by a cysteine knot will be constructed and added to a solution of single-stranded peptide consisting of tripeptide repeats known to form hyperstable triple helices. Binding of the single strands to the host will be assayed by fluorescence anisotropy and ultracentrifugation. To extend the effective reach of the single strands across a wound, multiple single-stranded peptides will be attached to the arms of a PAMAMdendrimer, and the ability of this multimeric structure to invade multiple collagen hosts will be assayed.