FISH Assay for Acute Myeloid Leukemia

描述(申请人摘要)：这项研究的目标是开发两个 荧光原位杂交(FISH)检测 T(8；21)(q22；q22)和inv(16)(P13q22)和相关的t(16；16)(p13；q22) 在急性髓系白血病(AML)的两个不同亚群中发现易位， M2和M4Eo。这些癌症的传统治疗方法是 化疗；然而，许多患者复发并对 治疗。诊断和治疗后随访目前由以下人员执行 核型、Southern杂交或聚合酶链式反应。然而，这些方法都有局限性。 在特异性和/或敏感性上。一种精确、可靠、简便的检测方法 需要检测癌细胞，特别是在治疗后的样本中。鱼 是对目前用于检测易位的方法的改进； 然而，没有经过临床测试的探针可用于检测AML。这款SBIR 该提案旨在开发新颖、灵敏和特异的鱼类检测方法， 在诊断和随访中明确检测携带核的易位 病人样本。 建议的商业应用： 拟议的研究将提供高度特异和灵敏的鱼类检测方法 用于癌症的急性髓系白血病(AML)的染色体易位 细胞遗传学参考实验室。

DESCRIPTION (Applicant's abstract): The goal of this research is to develop two fluorescence in situ hybridization (FISH) assays for detecting the t(8;21)(q22;q22) and inv(16)(p13q22) and related t(16;16)(p13;q22) translocations found in two different subsets of acute myeloid leukemia (AML), M2 and M4Eo, respectively. These cancers are traditionally treated by chemotherapy; however, many patients relapse and become refractory to treatment. Diagnosis and post treatment follow-up are currently performed by karyotype, Southern blotting or PCR. These methods, however, have limitations in specificity and/or sensitivity. A precise, reliable and simple method for detecting cancer cells, especially in post-treatment specimens, is needed. FISH is an improvement over current methods used for detecting translocations; however, no clinically tested probes are available for detecting AML. This SBIR proposal aims to develop novel, sensitive and specific FISH assays that will unambiguously detect translocation carrying nuclei in diagnostic and follow up patient samples. PROPOSED COMMERCIAL APPLICATION: The proposed research will provide highly specific and sensitive FISH assays for detecting chromosomal translocations present in Acute Myeloid Leukemia (AML) for use in cancer cytogenetic reference laboratories.