MOLECULAR PROBES FOR BURKITT'S AND MANTLE CELL LYMPHOMA

申请人？本研究的目的是开发两种 荧光原位杂交（FISH）检测 t（11;14）（q13;q32）和t（8;14）（q24;q32）染色体易位 在曼特尔和伯基特街相遇s B细胞淋巴瘤。这些肿瘤 传统上通过放射和化学疗法治疗;然而， 复发并变得难以治疗。诊断和治疗后 通过流式细胞术、核型分析、Southern印迹或 PCR，其中每一种都具有特异性和/或灵敏度的限制。 因此，另一种精确、可靠、简便的检测肿瘤细胞的方法 特别是在标本的后处理中是必不可少的。这种方法就是FISH。 不幸的是，没有临床测试的探针可用于检测 不是曼托就是伯基特s淋巴瘤。在第一阶段的申请中，我们建议 开发基于BAC的探针以检测诊断中的易位携带核 并追踪病人样本每个染色体的探针将被标记 用不同的颜色，并将明确识别两个正常和 每个细胞核中有两条重排的染色体，从而提供了高特异性 和敏感性。 拟定商业应用： 拟议的研究将提供FISH检测，以检测t（11;14）（q13;q32）和 t（8;14）（q24;q32）染色体易位在套细胞淋巴瘤和 伯基特淋巴瘤，分别用于癌症细胞遗传学参考实验室。

APPLICANT?S DESCRIPTION: The goal of this research is to develop two fluorescence in situ hybridization (FISH) assays for the detection of the t(11;14)(q13;q32) and the t(8;14)(q24;q32) chromosomal translocations encountered in Mantle and Burkitt?s B-cell Lymphoma respectively. These tumors are traditionally treated by radiation and chemotherapy; however many patients relapse and become refractory to treatment. Diagnosis and post-treatment follow-up are performed by flow cytometry, karyotype, Southern Blotting, or PCR, each of which has limitations of specificity and/or sensitivity. Therefore, another precise, reliable, and easy method to detect tumor cells especially in the post-treatment specimens is essential. Such a method is FISH. Unfortunately, no clinically tested probes are available for the detection of either Mantle or Burkitt?s Lymphoma. In this Phase I application, we propose to develop BAC-based probes to detect translocation carrying nuclei in diagnostic and follow up patient samples. The probes for each chromosome will be labeled with a different color and will unambiguously identify the two normal and the two rearranged chromosomes in each nucleus, thereby providing high specificity and sensitivity. PROPOSED COMMERCIAL APPLICATION: The proposed research will provide FISH assays to detect the t(11;14)(q13;q32) and t(8;14)(q24;q32) chromosomal translocations encountered in Mantle Cell Lymphoma and Burkitt's Lymphoma, respectively, for use in cancer cytogenetic reference laboratories.