Characterisation of mechanical, physical and biological properties of biofilms under industrial processing conditions

细菌生物膜为微生物提供了对外部环境的物理机械保护，需要使用苛刻的化学物质和研磨清洗来控制它们。这一保护机制在各种产品的生产中具有广泛的影响，包括为卫生设计的产品(口腔、家庭清洁和织物卫生)以及它们在家庭中的功效，在家庭中也可以体验到生物膜。目前，制造产品可能会受到细菌生物膜污染的影响，导致生产中断，并给工业带来巨大的清洁和消毒经济成本。在高流量条件下形成的生物膜还没有从物理和生物相结合的角度研究在这些压力下发生的变化。在这个项目中，将评估剪切和流动对生物膜物理和机械性能的影响，将这些因素与新陈代谢和细胞活动的变化联系起来。所产生的模型生物膜将用于研究假单胞菌的遗传、转录和代谢反应。由于制造和清洁过程中施加的压力。这些实验将为在各种条件下预防和消除假单胞菌生物被膜形成的最有效方法提供数据，以期设计更有效的预防措施

Bacterial biofilms provide a physico-mechanical protection of microbes to the external environment, requiring the use of harsh chemicals and abrasive cleaning for their control. This protective mechanism has broad impact in the manufacturing of a wide range of products including those designed for hygiene (oral, household cleaning and fabric hygiene) as well as their efficacy within the home, where biofilms can also be experienced. Currently, manufacturing products can be subject to problems with bacterial biofilm contamination leading to production interruption and significant economic cost to industry for cleaning and sanitisation. Biofilms formed under high flow conditions have not been studied in relation to the changes that occur in response to these stresses from a combined physical and biological perspective. The impact of shear and flow on the physical and mechanical properties of the biofilm will be evaluated in this project, relating these factors to changes in metabolism and cellular activity. The model biofilms generated will be used to investigate the genetic, transcriptomic and metabolic responses of Pseudomonas spp. to stresses imposed in manufacturing and cleaning processes. These experiments will provide data which will inform the most effective means to prevent and remove biofilm formation by Pseudomonas in a range of conditions, with a view to designing more effective remed