Core--Cell processing

细胞处理核心的目的是获取和提供人类血细胞，作为代谢疾病基因治疗计划项目赠款的一部分进行研究。通过这个核心项目提供的细胞包括外周血干细胞、单核细胞和脐带血细胞。这些细胞将从正常捐赠者或患者那里大规模获得，并以从捐赠者获得的组合物或在实验室处理以分离淋巴细胞或CD34+细胞等亚群的形式提供。细胞处理核心还将提供克隆分析的体外细胞培养服务，用于测量和评估已按照该计划项目的规定进行基因转移操作的造血祖细胞。随着项目走向临床应用，该项目将在圣保罗校区的GMP设施，即分子和细胞治疗设施，为临床试验提供同样的服务。

The purpose of the Cell Processing Core is to obtain and provide human blood cells for investigations being carried out as part of the Gene Therapy for Metabolic Disorders Program Project Grant. Cells that will be provided through this Core project include peripheral blood stem cells, mononuclear cells, and umbilical cord blood cells. These cells will be obtained on a large scale from either normal donors or patients and provided in the composition obtained from the donor or processed in the laboratory to isolate subpopulations such as lymphocytes or CD34+ cells. The Cell Processing Core will also provide in-vitro cell culture of clonogenic assay services for the measurement and evaluation of hematopoietic progenitor cells that have undergone gene transfer manipulations as specified in this program project. As projects move toward clinical application, the project will supply these same services for clinical trials at the GMP facility on the St. Paul Campus, the Molecular and Cellular Therapeutics Facility.