PROPERTIES OF SINGLE ASSEMBLIES OF LIGHT HARVESTERS LH1 & LH2

我们正在研究光漂白和转移动力学 黄体生成素复合体和β-二肽的组装 可逆地从他们身上提取出来。每个LH2包含18个 细菌叶绿素(B850)和9种捕收剂(B800)。这个 荧光寿命、偏振、相关时间和漂白 在各种条件下都在研究动力学。初级阶段 目标包括对长时间尺度蛋白质动力学的测量。 极化实验揭示了构象动力学 与非聚合体LH2组件中的激子迁移有关。 此外，他们还表示，单一程序集不是 电子圆形的，如从X射线衍射中所预期的 学习。

We are examing the photobleaching and transfer dynamics in single assemblies of the LH complexes and the ??-dipeptides that can be reversibly extracted from them. Each LH2 contains eighteen bacteriochlorophylls (B850) and nine collectors (B800). The fluorescence lifetimes, polarization, correlation times and bleaching kinetics are being studied under a variety of conditions. The primary goals include measurements of long time scale protein dynamics. Polarization experiments have revealed conformational dynamics associated with excitonic migration in the nonameric LH2 assembly. Furthermore, they have indicated that the single assemblies are not circular electronically as would be expected from X-ray diffraction studies.