Zeiss Axiovert 2000 for Live Cell Imaging

描述（由申请人提供）：在本申请中，我们建议增强 纽约大学新建立的核心显微镜设施 墨西哥健康科学中心（UNM HSC）通过收购活细胞 成像系统拟议的仪器将与我们的 现有的蔡司LSM 510和传统的数字成像设备。的 要求的仪器包括一个新的倒置显微镜（Axiovert 200） 配备了自动滤光轮和快门控制， 轴电动舞台。通过微观观察提供环境控制 来自Bioptechs的腔室、目标加热器和灌注设备。用于图像 我们计划为Axiovert 200配备数字摄像机， 将我们现有的LSM51O扫描头转移到Axiovert上的基座端口安装 200. LSM 51 O扫描头配备有三个激光器（Ar、绿色HeNe和红色 HeNe），并且能够使许多活细胞探针成像， 自体荧光蛋白变体。对于定量和图像分析，我们 请求将生理学软件包安装在我们的Zeiss LSM 5上 10.三维成像软件已经安装， 添加生理学软件包后，系统将执行4D成像， 允许跟踪和量化细胞器的运动，以及 荧光共振能量转移（FRET）测量和定量 光漂白后的荧光恢复（FRAP）实验。请求的 数字摄像机将与现有的数字视频成像集成 具有法师捕获和分析能力工作站。的 拟议仪器的购置将为七个主要用户服务 和几个小用户，并将提供这些独立的调查人员先进的 活细胞成像能力对于NIH资助的生物医学 research.活细胞研究将用于研究细胞内膜 转运，膜微区的时间组装，基因调控 表达和宿主病原体相互作用。

DESCRIPTION (provided by applicant): In this application we propose to enhance the newly established Core Microscopy Facilities at the University of New Mexico Health Sciences Center (UNM HSC) through the acquisition of a live cell imaging system. The proposed instrumentation will be integrated with our existing Zeiss LSM 510 and conventional digital imaging equipment. The requested instrumentation includes a new inverted microscope (Axiovert 200) equipped with automated filter wheels and shutter controls, and a fully x, y, z axis motorized stage. Environmental control is provided by micro-observation chambers, objective heaters and perfusion equipment from Bioptechs. For image capture we plan to equip the Axiovert 200 with a digital video camera or transfer our existing LSM51O scanhead to the baseport mount on the Axiovert 200. The LSM51O scanhead is equipped with three lasers (Ar, green HeNe and red HeNe) and is capable of imaging a number of live cell probes and autofluorescent protein variants. For quantification and image analysis we are requesting the Physiology software package to be installed on our Zeiss LSM5 10. Three dimensional imaging software is already installed and with the addition of the Physiology software package the system will perform 4D imaging, allow tracing and quantification of organellar movements, as well as fluorescence resonance energy transfer (FRET) measurements and quantification of fluorescence recovery after photobleaching (FRAP) experiments. The requested digital video camera will be integrated with an existing digital video imaging workstation complete with mage capture and analysis capabilities. The acquisition of the proposed instrumentation will serve seven principal users and several minor users and will offer these independent investigators advanced live cell imaging capabilities important for their NIH-funded biomedical research. Live cell studies will be used to investigate intracellular membrane transport, temporal assembly of membrane microdomains, the regulation of gene expression and host pathogen interactions.