Mass Spectrometer

描述(由申请人提供)：本提案要求资金用于 购买微质量Q-TOF Ultima全球混合四极杆/正交 分光计及其操作所需的附件。这件乐器是 之所以选择它，是因为它提供的功能(通过要安装的选项) MALDI和Q-TOF仪器在不影响质量的情况下 Q-TOF的分辨率、质量精度或电喷灵敏度。这 需要将仪器作为当前质谱计的升级 仪器可在西弗吉尼亚大学获得。附近没有类似的仪器。 设施。这台仪器将成为蛋白质组学/质谱学的核心仪器 将支持多个正在进行的NIH项目的光谱分析设施(表1) 以及一笔科布雷补助金(16440卢比)，将支持五名初级调查人员。 虽然Cobre蛋白质组学/MS核心将与可用的芬兰人一起启动 LCQ-DECA MS，此仪器有许多限制(详细说明如下)， 不宜长期使用。因此，这一升级对于 蛋白质组学/MS核心设施的持续使用。除 调查人员列出了证明这一工具的理由，预计随着 人们对科学中蛋白质组学的兴趣与日俱增，核心设施将 看到越来越多的用法。

DESCRIPTION (provided by applicant): This proposal requests the funds to purchase a Micromass Q-TOF Ultima Global Hybrid Quadrupole/orthogonal spectrometer and accessories necessary for its operation. This instrument was chosen because it provides the capabilities (through options to be installed) of both a MALDI as well as a Q-TOF instrument without compromising the mass resolution, mass accuracy or electrospray sensitivity of the Q-TOF. This instrument is requested as a upgrade to the current mass spectrometer instruments available at WVU. No comparable instruments are available in nearby facilities. This instrument will be the core instrument for a proteomics/mass spectrometry facility that will support several on-going NIH projects (Table 1) as well as a COBRe grant (RR16440) that will support five junior investigators. While the COBRe proteomics/MS core will be initiated with an available Finnigan LCQ -DECA MS, this instrument has many limitations (detailed below) that make it unsuitable for long-term use. Thus, this upgrade is essential to the continued utility of the proteomics/MS core facility. In addition to the investigators listed to justify this instrument, it is anticipated that with the increasing interest in proteomics in science, that the core facility will see more and more use.