A Research Grade MALDI TOF Mass Spectrometer

俄勒冈州立大学(OSU)化学系和环境健康科学中心(EHSC)申请资金购买新的高性能MALDI飞行时间质谱仪系统。为了适应俄勒冈州州立大学和俄勒冈州研究界对一系列MALDI质谱分析的快速增长的需求，该仪器是必要的。俄亥俄州立大学质谱学设施中现有的MALDI仪器不具备支持这一需求所需的灵敏度、分辨率、质量精度和样品吞吐量。特别是，现有文书不能提供本提案中被指定为主要用户的调查员所要求的信息的质量或数量。新仪器将用于制作蛋白质的酶和化学消化产生的多肽的质谱图，以努力识别蛋白质，获得序列标签以帮助识别蛋白质和蛋白质中的磷酸化或其他此类修饰位置，监测折叠中间体和由于修饰和配体相互作用而产生的构象状态的研究中氢和氢的交换，以及监测双链DNA中由于构象效应而导致的胞嘧啶的自发脱氨基。所有这些应用都将大大有利于NIH支持的研究。最后，新的质谱仪将支持大量校园内外的小用户，他们是那些在研究中寻求高性能MALDI分析的人之一。

The Department of Chemistry and the Environmental Health Sciences Center (EHSC) at Oregon State University (OSU) request funds to purchase a new high performance MALDI time-of-flight mass spectrometer system. This instrument is needed to accommodate the rapidly growing demand at OSU and the Oregon research community at large for a range of MALDI mass spectrometric analyses. The existing MALDI instrumentation in OSU's Mass Spectrometry Facility does not have the sensitivity, resolving power, mass accuracy, and sample throughput required to support this demand. In particular, the existing instruments are not capable of providing either the quality or quantity of information sought by the investigators designated as major users in this proposal. The new instrument would be used to produce mass- maps of peptides produced by enzymatic and chemical digestion of proteins in efforts to identify the proteins, to obtain sequence tags to aid in the identification of proteins and of sites of phosphorylation or other such modifications within the proteins, to monitor the exchange of hydrogen and deuterium in studies on folding intermediates and conformational states resulting from modifications and ligand interactions, and to monitor the spontaneous deamination of cytosine in double-stranded DNA due to conformational effects. All of these applications would substantially benefit research supported by NIH. Finally, the new mass spectrometer would support a large number of minor users both on- and off-campus who are among those seeking high performance MALDI analysis in their research.