Beckman Coulter BioMek 2000 Workstation and Accessories

描述(申请人提供)：用于基于流式细胞术的自动化 免疫功能分析，购买Beckman Coulter需要资金 BIOMEK 2000系统将设在格莱斯通/加州大学旧金山分校的核心免疫学 旧金山总医院的实验室。在核心实验室。一种流动 基于细胞学的检测系统已经被开发和优化，以检测 HIV-1和巨细胞病毒(CMV)对CD4+和CD8+T细胞的特异性应答 在HIV-1感染患者的队列中。与MHC I类四聚体分析不同， 这种细胞因子流式细胞术(CFC)可以用来检测T细胞 在未定义抗原表位的背景下的反应 未知的MHC背景。相关的检测方法正在开发中 人类CD4+和CD8+T细胞对其他细胞的特异性反应 与艾滋病相关的机会性感染，包括由分枝杆菌引起的感染 结核、禽类分枝杆菌复合体、卡氏肺孢子虫和人类 乳头状瘤病毒。加州大学旧金山分校的许多研究人员已经将氯氟化碳纳入他们的 研究方案；因此，在核心进行的氟氯化碳分析的次数 实验室正在稳步快速增长。跟上对…的需求 这项服务，以提高安全性和可靠性，并进行实验 这将是不切实际或不可能手动执行的，因此有必要 使cfc自动化。结合已经到位的工具，拟议的 仪器系统将使氟氯化碳的自动化和进一步发展成为可能 化验，显著加强和提高核心能力 免疫学实验室为许多Gladstone/UCSF调查人员提供支持 在艾滋病研究方面。此外，这些自动化化验将用于 从事其他研究领域的科学家和来自其他领域的科学家 在该国部分地区(例如，通过艾滋病临床试验小组)。这个 选定的主要用户的科学兴趣和专业知识包括广泛的 生物学的光谱和对免疫功能分析的统一关注； 因此，仪器系统将支持广泛的项目。 在以下背景下评估T细胞反应：1)HIV-1 疫苗，2)抗逆转录病毒治疗失败，3)结构化治疗 中断，4)交叉分支识别，5)CTL动力学，6)宫颈HPV 不典型增生，7)卡氏肺孢子虫感染，8)实体器官移植， 9)脱氢表雄酮“替代疗法”，10)脑脊液细胞增多症。总而言之，这是 建议的仪器系统是NIH资助的几个项目成功的关键 调查和研究人员，将为 Gladstone/加州大学旧金山分校的研究社区，并将在 未来。

DESCRIPTION (provided by applicant): For the automation of flow cytometry-based assays of immune function, funding is requested to purchase a Beckman Coulter BioMek 2000 system, to be located in the Gladstone/UCSF Core Immunology Laboratory at San Francisco General Hospital. In the Core Laboratory. a flow cytometry-based assay system has been developed and optimized to detect specific CD4+ and CD8+ T cell responses to HIV-1 and to cytomegalovirus (CMV) in cohorts of HIV-1-infected patients. Unlike the MHC Class I tetramer assay, this ?cytokine flow cytometry (CFC) assay can be used to detect T cell responses against undefined antigenic epitopes in the context of uncharacterized MHC backgrounds. Related assays are being developed for the assessment of specific human CD4+ and CD8+ T cell responses to other AIDS-related opportunistic infections, including those caused by Mvcobacterium tuberculosis, the Mvcobacterium avium complex, Pneumocvstis carinii, and human papilloma virus. Many investigators at UCSF have incorporated CFC into their research protocols; consequently the number of CFC assays performed in the Core Laboratory is steadily and rapidly increasing. To keep up with the demand for this service, to improve safety and reliability, and to perform experiments that would be impractical or impossible to perform manually, it is necessary to automate CFC. Combined with the instrumentation already in place, the proposed instrument system will allow for the automation and further development of CFC assays, significantly strengthening and enhancing the ability of the Core Immunology Laboratory to support the many Gladstone/UCSF investigators engaged in HIV research. In addition, these automated assays will be made available to scientists engaged in other areas of research and to scientists from other parts of the country (e.g., through the AIDS Clinical Trials Group). The scientific interests and expertise of the selected major users include a broad spectrum of biology and a unifying focus on the analysis of immune function; consequently, the instrument system will support a broad range of projects involving the evaluation of T cell responses in the context of: 1) HIV-1 vaccines, 2) antiretroviral therapy failure, 3) structured treatment interruption, 4) cross clade recognition, 5) CTL dynamics, 6) HPV in cervical dysplasia, 7) Pneumocystis carinii infection, 8) solid organ transplantation, 9) "alternative therapy" with DHEA, and 10) CSF pleocytosis. In summary, this proposed instrument system is pivotal to the success of several NIH-funded investigations and researchers, will provide a critical resource to the Gladstone/UCSF research community, and will spark innovative research in the future.